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Hydroxyl radical footprinting of fluorescently labeled DNA

Hydroxyl radical footprinting of fluorescently labeled DNA Footprinting is one of the simplest and most accurate approaches to investigate structure and interaction of biopolymers. It is based on the more difficult accessibility of intra- and intermolecular contacts for external damaging agents. According to this method, one end of polymer molecules is labeled before a sample is incubated with a damaging agent. The distribution of split products is used to conclude on the accessibility of different polymer regions under specific conditions. A variety of enzymatic and chemical splitting agents are used for footprinting. Currently, the highest temporal and spatial resolution without profound specificity to a nucleotide sequence can be reached with the use of hydroxyl radicals. A new variant of this approach, which suggests the use of DNA fluorescent labeling together with the present-day quantitative analysis, will allow extending the method’s boundaries. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Moscow University Biological Sciences Bulletin Springer Journals

Hydroxyl radical footprinting of fluorescently labeled DNA

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References (15)

Publisher
Springer Journals
Copyright
Copyright © 2016 by Allerton Press, Inc.
Subject
Life Sciences; Biochemistry, general; Cell Biology; Life Sciences, general; Plant Sciences; Zoology
ISSN
0096-3925
eISSN
1934-791X
DOI
10.3103/S0096392516020036
Publisher site
See Article on Publisher Site

Abstract

Footprinting is one of the simplest and most accurate approaches to investigate structure and interaction of biopolymers. It is based on the more difficult accessibility of intra- and intermolecular contacts for external damaging agents. According to this method, one end of polymer molecules is labeled before a sample is incubated with a damaging agent. The distribution of split products is used to conclude on the accessibility of different polymer regions under specific conditions. A variety of enzymatic and chemical splitting agents are used for footprinting. Currently, the highest temporal and spatial resolution without profound specificity to a nucleotide sequence can be reached with the use of hydroxyl radicals. A new variant of this approach, which suggests the use of DNA fluorescent labeling together with the present-day quantitative analysis, will allow extending the method’s boundaries.

Journal

Moscow University Biological Sciences Bulletin – Springer Journals

Published: Jul 13, 2016

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