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Hydrolysis of Soybean and Rapeseed Proteins with Enzyme Complex Extracted from the Pyloric Caeca of the Cod

Hydrolysis of Soybean and Rapeseed Proteins with Enzyme Complex Extracted from the Pyloric Caeca... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 2, pp. 165–172. © Pleiades Publishing, Inc., 2019. Russian Text © D.V. Zinchenko, T.A. Muranova, L.A. Melanyina, A.I. Miroshnikov, 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 2, pp. 172–180. Hydrolysis of Soybean and Rapeseed Proteins with Enzyme Complex Extracted from the Pyloric Caeca of the Cod a, a a a D. V. Zinchenko *, T. A. Muranova , L. A. Melanyina , and A. I. Miroshnikov Biotechnological Department, Moscow State University, Moscow, 119991 Russia *e-mail: zdv@bibch.ru Received April 12, 2018; revised September 3, 2018; accepted September 25, 2018 Abstract—Hydrolysis of soybean and rapeseed proteins with extract from cod pyloric caeca has been per- formed. The hydrolysates were analyzed by SDS-PAGE, HPLC, amino acid analysis, and mass spectrome- try. The degree of hydrolysis depended on the substrate/enzyme extract ratio, hydrolysis time, temperature, and protein concentration. The hydrolysis of soybean and rapeseed proteins at a substrate/enzyme extract ratio of 1 : 20 and a hydrolysis time of 20 h at 37°C led to complete cleavage of intact proteins. The soluble part of the rapeseed protein hydrolysate consisted of oligopeptides with a molecular weight of less than 14 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Hydrolysis of Soybean and Rapeseed Proteins with Enzyme Complex Extracted from the Pyloric Caeca of the Cod

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References (2)

Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819020182
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 2, pp. 165–172. © Pleiades Publishing, Inc., 2019. Russian Text © D.V. Zinchenko, T.A. Muranova, L.A. Melanyina, A.I. Miroshnikov, 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 2, pp. 172–180. Hydrolysis of Soybean and Rapeseed Proteins with Enzyme Complex Extracted from the Pyloric Caeca of the Cod a, a a a D. V. Zinchenko *, T. A. Muranova , L. A. Melanyina , and A. I. Miroshnikov Biotechnological Department, Moscow State University, Moscow, 119991 Russia *e-mail: zdv@bibch.ru Received April 12, 2018; revised September 3, 2018; accepted September 25, 2018 Abstract—Hydrolysis of soybean and rapeseed proteins with extract from cod pyloric caeca has been per- formed. The hydrolysates were analyzed by SDS-PAGE, HPLC, amino acid analysis, and mass spectrome- try. The degree of hydrolysis depended on the substrate/enzyme extract ratio, hydrolysis time, temperature, and protein concentration. The hydrolysis of soybean and rapeseed proteins at a substrate/enzyme extract ratio of 1 : 20 and a hydrolysis time of 20 h at 37°C led to complete cleavage of intact proteins. The soluble part of the rapeseed protein hydrolysate consisted of oligopeptides with a molecular weight of less than 14

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Applied Biochemistry and Microbiology – Springer Journals

Published: May 24, 2019

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