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HPTLC-MS analysis of melamine in milk: standardization and validation

HPTLC-MS analysis of melamine in milk: standardization and validation Melamine containing large amount of nitrogen may be deliberately added to milk in order to increase the protein content for economic adulteration in food stuffs. High performance thin layer chromatography-mass spectrometry (HPTLC-MS)-based method of quantification of melamine has been developed using Silica gel 60 F254s HPTLC plates, with optimized mobile phase iso-propanol/dichloromethane/water, 5:2.5:3, v/v/v in a twin trough chamber saturated (5 min) at pH 6.8. Validation was carried out by testing its specificity, linearity (1–50 μg), accuracy (95.908–98.369%), inter- and intraday precision (1.578 and 1.670%), respectively, limits of detection (2.688 ng) and quantification (8.146 ng). HPTLC in combination with MS has been found suitable for routine analysis of melamine added as adulterant to market-purchased milk samples. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Dairy Science & Technology Springer Journals

HPTLC-MS analysis of melamine in milk: standardization and validation

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Publisher
Springer Journals
Copyright
Copyright © 2014 by INRA and Springer-Verlag France
Subject
Chemistry; Food Science; Agriculture; Microbiology
ISSN
1958-5586
eISSN
1958-5594
DOI
10.1007/s13594-014-0204-3
Publisher site
See Article on Publisher Site

Abstract

Melamine containing large amount of nitrogen may be deliberately added to milk in order to increase the protein content for economic adulteration in food stuffs. High performance thin layer chromatography-mass spectrometry (HPTLC-MS)-based method of quantification of melamine has been developed using Silica gel 60 F254s HPTLC plates, with optimized mobile phase iso-propanol/dichloromethane/water, 5:2.5:3, v/v/v in a twin trough chamber saturated (5 min) at pH 6.8. Validation was carried out by testing its specificity, linearity (1–50 μg), accuracy (95.908–98.369%), inter- and intraday precision (1.578 and 1.670%), respectively, limits of detection (2.688 ng) and quantification (8.146 ng). HPTLC in combination with MS has been found suitable for routine analysis of melamine added as adulterant to market-purchased milk samples.

Journal

Dairy Science & TechnologySpringer Journals

Published: Dec 24, 2014

References