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Heterogeneity in phagocytic and oxidative burst activities of neutrophils and neonatal calves

Heterogeneity in phagocytic and oxidative burst activities of neutrophils and neonatal calves In vitro experiments were conducted to determine the influence of source of neutrophils (individual calves) and calf age on phagocytic and oxidative burst activities of neutrophils isolated from 106 clinically healthy Holstein-Friesian calves. Calves were categorised by age into four groups: group A, 3–7 days; group B, 8–14 days; group C, 15–21 days or group D, 22–31 days. Neutrophils were isolated (purity >90%, viability >97%) from EDTA-anticoagulated blood. Aliquots of 2 x 106 neutrophils were incubated at 37°C for 30 min with fluorescein-labelledEscherichia coli 0111:134 J5,E. coli 0157:117 andSalmonella dublin, and with pooled bovine serum as the opsonin. Percentage phagocytosis and averaged number of intracellular bacteria per neutrophil were evaluated by fluorescence microscopy. Mean channel intensity was determined by flow cytometry using dichlorofluorescein diacetate and phorbol myristate acetate to evaluate 11202 production, a measure of oxidative burst. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Heterogeneity in phagocytic and oxidative burst activities of neutrophils and neonatal calves

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References (51)

Publisher
Springer Journals
Copyright
Copyright © 1997 by Springer-Verlag London Limited
Subject
Medicine & Public Health; Hematology; Oncology
eISSN
1433-2973
DOI
10.1007/BF01320996
Publisher site
See Article on Publisher Site

Abstract

In vitro experiments were conducted to determine the influence of source of neutrophils (individual calves) and calf age on phagocytic and oxidative burst activities of neutrophils isolated from 106 clinically healthy Holstein-Friesian calves. Calves were categorised by age into four groups: group A, 3–7 days; group B, 8–14 days; group C, 15–21 days or group D, 22–31 days. Neutrophils were isolated (purity >90%, viability >97%) from EDTA-anticoagulated blood. Aliquots of 2 x 106 neutrophils were incubated at 37°C for 30 min with fluorescein-labelledEscherichia coli 0111:134 J5,E. coli 0157:117 andSalmonella dublin, and with pooled bovine serum as the opsonin. Percentage phagocytosis and averaged number of intracellular bacteria per neutrophil were evaluated by fluorescence microscopy. Mean channel intensity was determined by flow cytometry using dichlorofluorescein diacetate and phorbol myristate acetate to evaluate 11202 production, a measure of oxidative burst.

Journal

Comparative Clinical PathologySpringer Journals

Published: Dec 7, 2004

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