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Haemorrheology of equine platelet-neutrophil aggregates

Haemorrheology of equine platelet-neutrophil aggregates The filterability of platelet-neutrophil aggregates through filters containing 5 μm pores was determined to evaluate the potential of plateletneutrophil aggregates to alter blood flow in the microvasculature. Incubation of platelet-neutrophil mixture with platelet-activating factor (PAF) and with 0.5 μM adenosine diphosphate (ADP) resulted in large platelet-neutrophil aggregates which rapidly plugged filter pores. Incubation of platelet-neutrophil mixtures with lower concentrations of ADP resulted in small platelet-neutrophil aggregates that contained only one neutrophil. Filtration of these samples resulted in a 1.3-, 3.2- and 3.7-fold increase in initial filtration pressure at ADP concentrations of 0.05, 0.15 and 0.25 μM, respectively. We conclude that platelet-neutrophil aggregates have reduced deformability and may alter blood flow in the microvasculature. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Haemorrheology of equine platelet-neutrophil aggregates

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Publisher
Springer Journals
Copyright
Copyright © 1999 by Springer-Verlag
Subject
Medicine & Public Health; Hematology; Oncology; Pathology
eISSN
1433-2973
DOI
10.1007/BF02585536
Publisher site
See Article on Publisher Site

Abstract

The filterability of platelet-neutrophil aggregates through filters containing 5 μm pores was determined to evaluate the potential of plateletneutrophil aggregates to alter blood flow in the microvasculature. Incubation of platelet-neutrophil mixture with platelet-activating factor (PAF) and with 0.5 μM adenosine diphosphate (ADP) resulted in large platelet-neutrophil aggregates which rapidly plugged filter pores. Incubation of platelet-neutrophil mixtures with lower concentrations of ADP resulted in small platelet-neutrophil aggregates that contained only one neutrophil. Filtration of these samples resulted in a 1.3-, 3.2- and 3.7-fold increase in initial filtration pressure at ADP concentrations of 0.05, 0.15 and 0.25 μM, respectively. We conclude that platelet-neutrophil aggregates have reduced deformability and may alter blood flow in the microvasculature.

Journal

Comparative Clinical Pathology – Springer Journals

Published: Mar 7, 2007

References

  • Platelet-neutrophil interactions. Possible relevance in the pathogenesis of thrombosis and inflammation
    Bazzoni, G; Dehana, E; Maschio, AD
  • Neutrophil depletion suppresses111In-labeled platelet accumulation in infarcted myocardium
    Bednar, M; Smith, B; Pinto, A
  • Pulmonary injury resulting from perfusion with stored bank blood in the baboon and dog
    Bennett, SH; Geelhoed, GW; Aaron, RK
  • Rheological responses of neutrophils to different types of stimulation
    Buttrum, SM; Drost, EM; MacNee, W
  • Red cell deformability and its relevance to biood flow
    Chien, S
  • GMP-140 mediates adheslon of stimulated platelets to neutrophils
    Hamburger, SA; McEver, RP
  • Intravascular platelet clumping in rabbits
    Hughes, A; Tonks, RS
  • Thrombosis and inflammation as multicellular processes: pathophysiologic significance of transcellular metabolism
    Marcus, A.
  • Leukocyte accumulation promoting fibrin deposition is mediated in vivo by P-selectin on adherent platelets
    Palabrica, T; Lobb, R; Furie, BC
  • Cardiopulmonary bypass induces leukocyte-platcleet adhesion
    Rinden, CS; Bonan, TL; Rinden, HM
  • Neutrophil accumulation on activated, surface-adherent platelets in flow is mediated by interaction of MAC-1 with fibrinogen bound to alpha-IIa-beta-3 and stimulated by platelet-activating factor
    Weber, C; Springer, TA
  • Detection of activated platelets and platelet-leukocyte aggregates in horses
    Weiss, DJ; Evanson, OA
  • Isolation of granulocytes and mononuclear cells from blood of dogs, cats, horses, and cows
    Weiss, DJ; Kraemer, R; Schmit, K
  • Filterability of densityseparated equine erythrocytes, neutrophils and mononuclear cells
    Weiss, DJ; Evanson, OA; Geor, RJ
  • The effects of furosemide and pentoxifylline on the flow properties of equine erythrocytes: in vitro studies
    Weiss, DJ; Evanson, OA; Geor, RJ
  • Effects of hematocrit and RBC deformability on pulmonary vascular pressures in perfused pony lungs
    Weiss, DJ; Richwagen, K; Evanson, OA
  • Effects of furosemide on hemorheologic alterations induced by incremental treadmill exercise in Thoroughbreds
    Weiss, DJ; Geor, RJ; Burger, K
  • Effects of pentoxifylline on hemorheologic alterations induced by incremental treadmill exercise in Thoroughbreds
    Weiss, DJ; Geor, RJ; Burger, K
  • Evaluation of platetet activation and platelet-neutrophil aggregates in ponies with alimentary laminitis
    Weiss, DJ; Evanson, OA; McClenahan, D
  • Evaluation of arginine-glycine-aspartate-containing peptides as inhibitors of equine platelet function
    Weiss, DJ; Evanson, OA; Wells, RE
  • A competitive inhibitor of platelet aggregation prevents experimental equine laminitis
    Weiss, DJ; Evanson, OA; McClenahan, D
  • Shear-induced platelet activation and platelet-neutrophils aggregates formation by equine platelets
    Weiss, DJ; Evanson, OA; McClenahan, D
  • Evaluation of platelet activation and platelet-neutrophil aggregates in Thoroughbreds undergoing near-maximal treadmill exercise
    Weiss, DJ; Evanson, OA; Fagliari, JJ
  • Mechanisms of stimulated neutrophils: cell stiffening induced retention in capillaries
    Worthen, GS; Schab, B; Elson, EL
  • Neutrophil rheologic changes in sepsis
    Yodice, PC; Astiz, ME; Kurian, BM