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ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 7, pp. 711–721. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2018, published in Biotekhnologiya, 2018, Vol. 34, No. 3, pp. 9–22. PROBLEMS AND PROSPECTS Genome Editing: Current State of Research and Application to Animal Husbandry a, a a N. A. Zinovieva *, N. A. Volkova , and V. A. Bagirov Ernst Federal Science Center for Animal Husbandry, Moscow oblast, Dubrovitsy village, 142132 Russia *e-mail: n_zinovieva@mail.ru Received April 1, 2018; revised April 1, 2018; accepted April 20, 2018 Abstract—The creation and development of genome editing (GE) technologies bring new opportunities for the genetic engineering of farm mammals and poultry. The present review characterizes GE systems based on ZFN, TALEN, and CRISPR/Cas9 and directions for their improvement in relation to farm animals. The f ields for the application of GE technologies in animal husbandry and poultry farming and the objectives and prospects for their further development are discussed. Keywords: genome editing, CRISPR/Cas9, farm animals DOI: 10.1134/S000368381907007X INTRODUCTION transferred embryos in cattle, sheep, goats, and pigs) to 1.0–2.0% in rabbits), it was almost completely The use of gene-engineering methods to modify replaced by somatic cell nuclear transfer (SCNT) [5]
Applied Biochemistry and Microbiology – Springer Journals
Published: Dec 5, 2019
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