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In suspensions of epididymal spermatozoa in vitro at +10°C and +37°C, all nuclei-containing and mitochondria-containing structures (normal spermatozoa, spermatozoa with the bent and coiled tails, complexes of head and neck) are with propidium iodide and rhodamine 123, respectively. Intracellular ATP concentration determined by a bioluminescent method in mitochondria-containing elements of suspension decreases (significantly faster at 37°C than at 10°C) up to a certain unchangeable level (2.5 × 10−8 M/l at 37°C and to 1.6 × 10−8 M/l at 10°C per 106 of mitochondria-containing elements). Mechanisms of spermatozoa destruction are discussed.
Moscow University Biological Sciences Bulletin – Springer Journals
Published: Jul 1, 2008
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