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Functional analysis of BT4 of Arabidopsis thaliana in resistance against Botrytis cinerea

Functional analysis of BT4 of Arabidopsis thaliana in resistance against Botrytis cinerea Botrytis cinerea is a fungus with a necrotrophic lifestyle attacking over 200 crop hosts worldwide, resulting in significant economic losses. At present, the molecular and cellular mechanisms involved in plant resistance to B. cinerea and their genetic control are poorly understood. The Arabidopsis BT4 gene was previously isolated by DDRT-PCR under B. cinerea infection. However a role for BT4 in defense signaling has not been described to date. Compared with wild-type (wt) Col-0, the loss-of-function mutant of BT4 showed increased susceptibility to B. cinerea and enhanced expression of some defense-related genes such as PR1, SOD1, PPO, PAL, POD and CAT. However, expression of other defense-related genes such as NPR1, PR4 and PDF1.2 were repressed in the mutant compared with wt plants. In addition, transgenic lines overexpressing BT4 restored resistance to B. cinerea. Taken together, our results indicate that BT4 play an important role in Arabidopsis in resistance to B. cinerea perhaps by regulating the expression of defense-related genes in response to SA and JA signaling pathways. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

Functional analysis of BT4 of Arabidopsis thaliana in resistance against Botrytis cinerea

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References (32)

Publisher
Springer Journals
Copyright
Copyright © 2013 by Australasian Plant Pathology Society Inc.
Subject
Life Sciences; Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1007/s13313-013-0202-6
Publisher site
See Article on Publisher Site

Abstract

Botrytis cinerea is a fungus with a necrotrophic lifestyle attacking over 200 crop hosts worldwide, resulting in significant economic losses. At present, the molecular and cellular mechanisms involved in plant resistance to B. cinerea and their genetic control are poorly understood. The Arabidopsis BT4 gene was previously isolated by DDRT-PCR under B. cinerea infection. However a role for BT4 in defense signaling has not been described to date. Compared with wild-type (wt) Col-0, the loss-of-function mutant of BT4 showed increased susceptibility to B. cinerea and enhanced expression of some defense-related genes such as PR1, SOD1, PPO, PAL, POD and CAT. However, expression of other defense-related genes such as NPR1, PR4 and PDF1.2 were repressed in the mutant compared with wt plants. In addition, transgenic lines overexpressing BT4 restored resistance to B. cinerea. Taken together, our results indicate that BT4 play an important role in Arabidopsis in resistance to B. cinerea perhaps by regulating the expression of defense-related genes in response to SA and JA signaling pathways.

Journal

Australasian Plant PathologySpringer Journals

Published: Mar 2, 2013

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