Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Fructose 1,6-bisphosphatase and aldolase B location in organs of sheep supplemented with nonprotein nitrogen

Fructose 1,6-bisphosphatase and aldolase B location in organs of sheep supplemented with... In ruminants, ammonia interferes in gluconeogenesis, reducing conversion of propionate to glucose. The importance of renal and hepatic gluconeogenesis in ruminants is well established, but cellular localization of the key gluconeogenic enzymes liver fructose-1,6-bisphosphatase (FBPase, EC.3.1.3.11) and aldolase B (AldoB, EC.4.1.2.13) in these organs and the potential contribution of other tissues in this process have not been investigated. The objective of this study was to determine the effect of nonprotein nitrogen (NPN) diets on the cellular localization of FBPase and AldoB in sheep organs. Eighteen sheep, allotted to three groups, were supplemented with NPN in the diet every 8 h for 18 days: Control (no NPN, 16.8 % crude protein (CP)), moderate NPN = 182 mg N/kg0.75 (22.9 % CP), and high NPN = 425 mg N/kg0.75 (30.7 % CP). Animals were slaughtered and pieces of organs were collected. Immunostaining for FBPase and AldoB expressions were examined by light microscopy, and co-localizations of these enzymes were examined with a confocal microscope. FBPase and AldoB were expressed in the cytoplasm of periportal hepatocytes, proximal tubular cells of the kidney, columnar absorptive cells of the duodenum, submucosa of the rumen, and β cells of Langerhans’ islets in the pancreas. Moreover, AldoB revealed to be located in the nucleus of hepatocytes. The localizations of both enzymes were not modified by NPN supplementation. In conclusion, FBPase and AldoB were detected in sheep tissues other than the liver and kidney, suggesting their potential contribution to gluconeogenesis or other functions in these tissues and their expressions were not changed by the inclusion of NPN in the diet. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Fructose 1,6-bisphosphatase and aldolase B location in organs of sheep supplemented with nonprotein nitrogen

Download PDF
5 pages

Loading next page...
 
/lp/springer-journals/fructose-1-6-bisphosphatase-and-aldolase-b-location-in-organs-of-sheep-udm6WzgsbE
Publisher
Springer Journals
Copyright
Copyright © 2013 by Springer-Verlag London
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1618-565X
DOI
10.1007/s00580-013-1744-2
Publisher site
See Article on Publisher Site

Abstract

In ruminants, ammonia interferes in gluconeogenesis, reducing conversion of propionate to glucose. The importance of renal and hepatic gluconeogenesis in ruminants is well established, but cellular localization of the key gluconeogenic enzymes liver fructose-1,6-bisphosphatase (FBPase, EC.3.1.3.11) and aldolase B (AldoB, EC.4.1.2.13) in these organs and the potential contribution of other tissues in this process have not been investigated. The objective of this study was to determine the effect of nonprotein nitrogen (NPN) diets on the cellular localization of FBPase and AldoB in sheep organs. Eighteen sheep, allotted to three groups, were supplemented with NPN in the diet every 8 h for 18 days: Control (no NPN, 16.8 % crude protein (CP)), moderate NPN = 182 mg N/kg0.75 (22.9 % CP), and high NPN = 425 mg N/kg0.75 (30.7 % CP). Animals were slaughtered and pieces of organs were collected. Immunostaining for FBPase and AldoB expressions were examined by light microscopy, and co-localizations of these enzymes were examined with a confocal microscope. FBPase and AldoB were expressed in the cytoplasm of periportal hepatocytes, proximal tubular cells of the kidney, columnar absorptive cells of the duodenum, submucosa of the rumen, and β cells of Langerhans’ islets in the pancreas. Moreover, AldoB revealed to be located in the nucleus of hepatocytes. The localizations of both enzymes were not modified by NPN supplementation. In conclusion, FBPase and AldoB were detected in sheep tissues other than the liver and kidney, suggesting their potential contribution to gluconeogenesis or other functions in these tissues and their expressions were not changed by the inclusion of NPN in the diet.

Journal

Comparative Clinical Pathology – Springer Journals

Published: Apr 24, 2013

References

  • Gluconeogenesis in dairy cows: the secret of making sweet milk from sour dough
    Aschenbach, JR; Kristensen, NB; Donkin, SS; Hammon, HM; Penner, GB
  • Histone 2B (H2B) expression is confined to a proper NAD+/NADH redox status
    Dai, RP; Yu, FX; Goh, SR; Chng, HW; Tan, YL; Fu, JL; Zheng, L; Luo, Y
  • Mice mutant for glucokinase regulatory protein exhibit decreased liver glucokinase: a sequestration mechanism in metabolic regulation
    Farrelly, D; Brown, KS; Tieman, A; Ren, J; Lira, SA; Hagan, D; Gregg, R; Mookhtiar, KA; Hariharan, N
  • Univalent cation activation of fructose 1,6-diphosphate
    Hubert, E; Villanueva, J; Gonzalez, AM; Marcus, F
  • A novel function of intestinal gluconeogenesis: central signaling in glucose and energy homeostasis
    Mithieux, G
  • Substrate utilization for hepatic gluconeogenesis is altered by increased glucose demand in ruminants
    Overton, TR; Drackley, JK; Ottemann-Abbamonte, CJ; Beauieu, AD; Emmert, LS; Clark, JH
  • Subcellular localization of aldolase B
    Sáez, DE; Slebe, JC
  • Nuclear localization of liver FBPase isoenzyme in kidney and liver
    Yanez, AJ; Bertinat, R; Concha, II; Slebe, JC
  • Novel expression of liver FBPase in Langerhans islets of human and rat pancreas
    Yanez, AJ; Bertinat, R; Spichiger, C; Carcamo, JG; Los Angeles Garcia, M; Concha, II; Nualart, F; Slebe, JC
  • Subcellular localization of liver FBPase is modulated by metabolic conditions
    Yanez, AJ; Garcia-Rocha, M; Bertinat, R; Droppelmann, C; Concha, II; Guinovart, JJ; Slebe, JC
  • Different involvement for aldolase isoenzymes in kidney glucose metabolism: aldolase B but not aldolase A colocalizes and forms a complex with FBPase
    Yanez, AJ; Ludwig, HC; Bertinat, R; Spichiger, C; Gatica, R; Berlien, G; Leon, O; Brito, M; Concha, II; Slebe, JC