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Freezing stallion semen in INRA96®-based extender improves fertility rates in comparison with INRA82

Freezing stallion semen in INRA96®-based extender improves fertility rates in comparison with INRA82 The chemical composition of freezing extenders plays a major role in sperm cell survival during cryopreservation. In this study we compared two extenders for freezing stallion semen: INRA82 extender (as a control) versus INRA96® extender, both supplemented with egg yolk and glycerol. INRA82 contains milk, whereas INRA96® is a chemically-defined extender developed for fresh semen storage at 4 °C or 15 °C. Semen from 3 stallions (7 ejaculates per stallion) was frozen in both extenders. In vitro analyses of post-thaw motility of sperm cells (computer-assisted analysis) and of membrane integrity (flow cytometry analysis) were performed. Then a fertility trial was conducted. Inseminations were conducted in a total of 84 mare cycles. INRA96® extender supplemented with egg yolk and glycerol significantly improved per-cycle pregnancy rates compared with ESTRA82 (71% versus 40%, p < 0.01). In agreement with these fertility results, membrane integrity was better preserved in INRA96® than in INRA82. In contrast, motility parameters were significantly higher in INRA82 than in INRA96®. Further research is needed to understand how INRA96® components protect sperm cells during the cryopreservation process and highly increase their fertility potential compared with INRA82 components. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Dairy Science & Technology Springer Journals

Freezing stallion semen in INRA96®-based extender improves fertility rates in comparison with INRA82

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Publisher
Springer Journals
Copyright
Copyright © 2008 by Springer S+B Media B.V.
Subject
Chemistry; Food Science; Agriculture; Microbiology
ISSN
1958-5586
eISSN
1958-5594
DOI
10.1051/dst:2008002
Publisher site
See Article on Publisher Site

Abstract

The chemical composition of freezing extenders plays a major role in sperm cell survival during cryopreservation. In this study we compared two extenders for freezing stallion semen: INRA82 extender (as a control) versus INRA96® extender, both supplemented with egg yolk and glycerol. INRA82 contains milk, whereas INRA96® is a chemically-defined extender developed for fresh semen storage at 4 °C or 15 °C. Semen from 3 stallions (7 ejaculates per stallion) was frozen in both extenders. In vitro analyses of post-thaw motility of sperm cells (computer-assisted analysis) and of membrane integrity (flow cytometry analysis) were performed. Then a fertility trial was conducted. Inseminations were conducted in a total of 84 mare cycles. INRA96® extender supplemented with egg yolk and glycerol significantly improved per-cycle pregnancy rates compared with ESTRA82 (71% versus 40%, p < 0.01). In agreement with these fertility results, membrane integrity was better preserved in INRA96® than in INRA82. In contrast, motility parameters were significantly higher in INRA82 than in INRA96®. Further research is needed to understand how INRA96® components protect sperm cells during the cryopreservation process and highly increase their fertility potential compared with INRA82 components.

Journal

Dairy Science & TechnologySpringer Journals

Published: May 21, 2011

References