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Flow cytometric determination of peripheral blood lymphocyte subpopulations and haematological values in the neonatal calf

Flow cytometric determination of peripheral blood lymphocyte subpopulations and haematological... Non-vaccinated Holstein female calves, 3–31 days old (n=106) were examined in order to determine peripheral blood lymphocyte subpopulations and haematological values. The relative populations of lymphocytes were determined using flow cytometry. Monoclonal antibodies were used to identify BoCD2+, BoCD4+, BoCD8+, B-cells,γ/δ T-cells, and monocytes/neutrophils, respectively. Complete blood counts were also determined. Calves were stratified into four groups by age (days): group A = 3–7 days, group B = 8–14 days, group C = 15–21 days and group D = 22–31 days. Group A calves had 30%–70% lower numbers of all lymphocyte subtypes compared to older calves. Group B calves had significantly lower numbers of B-cells than calves in group D, but were not different from group C. Calves in groups B and C had significantly lowerγ/δ T-cell counts than calves in group D. All other group comparisons of lymphocyte subtypes and the ratios of BoCD4+ to BoCD8+ T-cells were not significantly different. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Flow cytometric determination of peripheral blood lymphocyte subpopulations and haematological values in the neonatal calf

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References (44)

Publisher
Springer Journals
Copyright
Copyright © 1997 by Springer-Verlag London Limited
Subject
Medicine & Public Health; Hematology; Oncology
eISSN
1433-2973
DOI
10.1007/BF01320993
Publisher site
See Article on Publisher Site

Abstract

Non-vaccinated Holstein female calves, 3–31 days old (n=106) were examined in order to determine peripheral blood lymphocyte subpopulations and haematological values. The relative populations of lymphocytes were determined using flow cytometry. Monoclonal antibodies were used to identify BoCD2+, BoCD4+, BoCD8+, B-cells,γ/δ T-cells, and monocytes/neutrophils, respectively. Complete blood counts were also determined. Calves were stratified into four groups by age (days): group A = 3–7 days, group B = 8–14 days, group C = 15–21 days and group D = 22–31 days. Group A calves had 30%–70% lower numbers of all lymphocyte subtypes compared to older calves. Group B calves had significantly lower numbers of B-cells than calves in group D, but were not different from group C. Calves in groups B and C had significantly lowerγ/δ T-cell counts than calves in group D. All other group comparisons of lymphocyte subtypes and the ratios of BoCD4+ to BoCD8+ T-cells were not significantly different.

Journal

Comparative Clinical PathologySpringer Journals

Published: Dec 7, 2004

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