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First experiences with cough sensitivity in model of allergic rhinitis induced in HDM-sensitized guinea pigs

First experiences with cough sensitivity in model of allergic rhinitis induced in HDM-sensitized... Buday et al. Clinical and Translational Allergy 2015, 5(Suppl 4):P8 http://www.ctajournal.com/content/5/S4/P8 POSTER PRESENTATION Open Access First experiences with cough sensitivity in model of allergic rhinitis induced in HDM-sensitized guinea pigs 1* 1 1 2 2 1 Tomas Buday , Silvia Gavliakova , Eva Kovacova , Juraj Mokry , Ivana Medvedova , Jana Plevkova From The 10th Symposium of Experimental Rhinology and Immunology of the Nose (SERIN 2015) Stockholm, Sweden. 19-21 February 2015 Background cough challenges with citric acid (0.4M) were performed. Nowadays, the vast majority of research focusing on Airway resistance was measured in vivo by Pennock’s cough in sensitive and/or hyperreactive airways is done method. on an animal model of guinea pigs intraperitoneally sen- sitized by ovalbumin. There are no objections to model Results animal – the neurophysiology and neuropharmacology of Provisionally obtained data show, that the cough their n. X are closest to humans. However, the choice of response in HDM-sensitized animals is similar to ovalbumin as model antigen together with the route of response of OVA sensitized animals (control vs. HDM administration remains questionable – allergy to chicken vs. OVA – 9 vs. 16 vs. 15. cough bursts/10min). Same eggs in humans manifests as food allergy, not respiratory similarity is observed in cough latency (control vs. HDM allergy. These limitations may represent possible obsta- vs. OVA – 180s vs. 86s vs. 80s). Airway resistance was cles in translation of results in daily clinical practice. increased, but statistical significance was not achieved. Therefore there is need to develop and validate new model of airway hypersensitivity, which would simulate Acknowledgement real-life conditions more closely and new model could Supported by Comenius University Grant No. UK/120/2014. improve translation of results. Most important indoor Authors’ details allergen for people are house dust mites (HDM), most Jessenius faculty of Medicine, Comenius University, Department of common species being D. pteronyssius and D. farinae. 2 Pathophysiology, Martin, Slovakia. Jessenius faculty of Medicine, Comenius Their allergenic potential is complex – it includes immu- University, Department of Pharmacology, Martin, Slovakia. nogenic epitopes, faecal pellets, lipopolysaccharides, beta- Published: 26 June 2015 glucans and chitin. Material and methods doi:10.1186/2045-7022-5-S4-P8 10 male guinea pigs (strain Dunkin-Hartley) were used to Cite this article as: Buday et al.: First experiences with cough sensitivity develop HDM model of airway hypersensitivity. Animals in model of allergic rhinitis induced in HDM-sensitized guinea pigs. Clinical and Translational Allergy 2015 5(Suppl 4):P8. were sensitized by 0.25% HDM aerosol (Greer Labs, USA), which they inhaled for 5 min over 5 days, followed by inhalation of 0.5% HDM in same protocol as before. Sensitization was confirmed by skin prick test (15µL; 0.5% HDM applied intradermally). In SPT positive ani- mals the symptoms of allergic rhinitis were induced by intranasal application of HDM (0.5%; 15µL) and the Jessenius faculty of Medicine, Comenius University, Department of Pathophysiology, Martin, Slovakia Full list of author information is available at the end of the article © 2015 Buday et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http:// creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/ zero/1.0/) applies to the data made available in this article, unless otherwise stated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical and Translational Allergy Springer Journals

First experiences with cough sensitivity in model of allergic rhinitis induced in HDM-sensitized guinea pigs

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Buday et al.
Subject
Medicine & Public Health; Allergology; Immunology
eISSN
2045-7022
DOI
10.1186/2045-7022-5-S4-P8
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Abstract

Buday et al. Clinical and Translational Allergy 2015, 5(Suppl 4):P8 http://www.ctajournal.com/content/5/S4/P8 POSTER PRESENTATION Open Access First experiences with cough sensitivity in model of allergic rhinitis induced in HDM-sensitized guinea pigs 1* 1 1 2 2 1 Tomas Buday , Silvia Gavliakova , Eva Kovacova , Juraj Mokry , Ivana Medvedova , Jana Plevkova From The 10th Symposium of Experimental Rhinology and Immunology of the Nose (SERIN 2015) Stockholm, Sweden. 19-21 February 2015 Background cough challenges with citric acid (0.4M) were performed. Nowadays, the vast majority of research focusing on Airway resistance was measured in vivo by Pennock’s cough in sensitive and/or hyperreactive airways is done method. on an animal model of guinea pigs intraperitoneally sen- sitized by ovalbumin. There are no objections to model Results animal – the neurophysiology and neuropharmacology of Provisionally obtained data show, that the cough their n. X are closest to humans. However, the choice of response in HDM-sensitized animals is similar to ovalbumin as model antigen together with the route of response of OVA sensitized animals (control vs. HDM administration remains questionable – allergy to chicken vs. OVA – 9 vs. 16 vs. 15. cough bursts/10min). Same eggs in humans manifests as food allergy, not respiratory similarity is observed in cough latency (control vs. HDM allergy. These limitations may represent possible obsta- vs. OVA – 180s vs. 86s vs. 80s). Airway resistance was cles in translation of results in daily clinical practice. increased, but statistical significance was not achieved. Therefore there is need to develop and validate new model of airway hypersensitivity, which would simulate Acknowledgement real-life conditions more closely and new model could Supported by Comenius University Grant No. UK/120/2014. improve translation of results. Most important indoor Authors’ details allergen for people are house dust mites (HDM), most Jessenius faculty of Medicine, Comenius University, Department of common species being D. pteronyssius and D. farinae. 2 Pathophysiology, Martin, Slovakia. Jessenius faculty of Medicine, Comenius Their allergenic potential is complex – it includes immu- University, Department of Pharmacology, Martin, Slovakia. nogenic epitopes, faecal pellets, lipopolysaccharides, beta- Published: 26 June 2015 glucans and chitin. Material and methods doi:10.1186/2045-7022-5-S4-P8 10 male guinea pigs (strain Dunkin-Hartley) were used to Cite this article as: Buday et al.: First experiences with cough sensitivity develop HDM model of airway hypersensitivity. Animals in model of allergic rhinitis induced in HDM-sensitized guinea pigs. Clinical and Translational Allergy 2015 5(Suppl 4):P8. were sensitized by 0.25% HDM aerosol (Greer Labs, USA), which they inhaled for 5 min over 5 days, followed by inhalation of 0.5% HDM in same protocol as before. Sensitization was confirmed by skin prick test (15µL; 0.5% HDM applied intradermally). In SPT positive ani- mals the symptoms of allergic rhinitis were induced by intranasal application of HDM (0.5%; 15µL) and the Jessenius faculty of Medicine, Comenius University, Department of Pathophysiology, Martin, Slovakia Full list of author information is available at the end of the article © 2015 Buday et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http:// creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/ zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Journal

Clinical and Translational Allergy – Springer Journals

Published: Jun 26, 2015

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