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Laurent Lamalice, F. Boeuf, J. Huot (2007)
Endothelial cell migration during angiogenesis.Circulation research, 100 6
S. Zigmond (1988)
[6] Orientation chamber in chemotaxisMethods in Enzymology, 162
Yu-Chih Chen, S. Allen, Patrick Ingram, R. Buckanovich, S. Merajver, E. Yoon (2015)
Single-cell Migration Chip for Chemotaxis-based Microfluidic Selection of Heterogeneous Cell PopulationsScientific Reports, 5
Scott Valastyan, R. Weinberg (2011)
Tumor Metastasis: Molecular Insights and Evolving ParadigmsCell, 147
Jan Schwarz, V. Bierbaum, J. Merrin, Tino Frank, R. Hauschild, T. Bollenbach, S. Tay, M. Sixt, M. Mehling (2016)
A microfluidic device for measuring cell migration towards substrate-bound and soluble chemokine gradientsScientific Reports, 6
Guilhem Velve-Casquillas, M. Berre, M. Piel, P. Tran (2010)
Microfluidic tools for cell biological research.Nano today, 5 1
A. Nauta, A. Nauta, G. Gurtner, M. Longaker (2011)
Wound healing and regenerative strategies.Oral diseases, 17 6
AnXiu Gao, Yunli Tian, Zhuanzhuan Shi, Ling Yu (2016)
A cost-effective microdevice bridges microfluidic and conventional in vitro scratch / wound-healing assay for personalized therapy validationBioChip Journal, 10
N. Kramer (2013)
10Mut. Res., 752
R. Riahi, Yongliang Yang, Donna Zhang, P. Wong (2012)
Advances in Wound-Healing Assays for Probing Collective Cell MigrationJournal of Laboratory Automation, 17
Paul Martin, Paul Martin, R. Nunan (2015)
Cellular and molecular mechanisms of repair in acute and chronic wound healingThe British Journal of Dermatology, 173
J. Fowler (1989)
Wound healing: an overview.Seminars in veterinary medicine and surgery, 4 4
S. Boyden (1962)
THE CHEMOTACTIC EFFECT OF MIXTURES OF ANTIBODY AND ANTIGEN ON POLYMORPHONUCLEAR LEUCOCYTESThe Journal of Experimental Medicine, 115
C. Justus, N. Leffler, M. Ruiz-Echevarría, Li Yang (2014)
In vitro cell migration and invasion assays.Journal of visualized experiments : JoVE, 88
D. Zicha, G. Dunn, A. Brown (1991)
A new direct-viewing chemotaxis chamber.Journal of cell science, 99 ( Pt 4)
S. Zigmond (1988)
Orientation chamber in chemotaxis.Methods in enzymology, 162
Fuqiang Nie, M. Yamada, J. Kobayashi, M. Yamato, A. Kikuchi, T. Okano (2007)
On-chip cell migration assay using microfluidic channels.Biomaterials, 28 27
Seok Chung, R. Sudo, Vernella Vickerman, I. Zervantonakis, R. Kamm (2010)
Microfluidic Platforms for Studies of Angiogenesis, Cell Migration, and Cell–Cell InteractionsAnnals of Biomedical Engineering, 38
C. Moraes, G. Mehta, S. Lesher‐Pérez, S. Takayama (2012)
Organs-on-a-Chip: A Focus on Compartmentalized MicrodevicesAnnals of Biomedical Engineering, 40
D. Zicha (1991)
769J. Cell. Sci., 99
Germán Reig, E. Pulgar, M. Concha (2014)
Cell migration: from tissue culture to embryosDevelopment, 141
M. Zhang, Hongjing Li, Huipeng Ma, J. Qin (2013)
A simple microfluidic strategy for cell migration assay in an in vitro wound‐healing modelWound Repair and Regeneration, 21
N. Jeon, H. Baskaran, S. Dertinger, G. Whitesides, L. Water, M. Toner (2002)
Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated deviceNature Biotechnology, 20
G. Broughton II (2006)
1ePlast. Reconstr. Surg., 117
P. Friedl, Katarina Wolf (2003)
Tumour-cell invasion and migration: diversity and escape mechanismsNature Reviews Cancer, 3
Abstract This work describes a microfludic cell culture device embedded with microstructured posts fabricated by a photolithography technique and a replica-molding technique. BALB/3T3 fibroblast cells were cultured inside the PDMS microfludic chip to form a confluent state, and an external pressure was applied to the top part of the microfluidic chip to remove the cells from the contact areas of the posts. The applied pressure was removed after the formation of wound areas in the cell layer, and the wound healing processes were investigated by monitoring the migration and proliferation of BALB/3T3 fibroblast cells. The function of the microfluidic chip actuated by an applied pressure was investigated by a fluorescent material, and the formation of the wound areas by an applied pressure was investigated by optical microscopy. After the formation of the wound in the cell layer, optical microscopic images of the cells at the same positions were captured by an optical microscope at intervals of 12 h in order to monitor the wound healing processes by migration and proliferation of the cells. Finally, the wound healing was quantitatively assessed by plotting a growth curve. In conclusion, because the microfluidic device developed in this work is very simple and easy to use, the device might be applicable to assessing the wound healing processes and monitoring the migration and proliferation of other cells.
BioChip Journal – Springer Journals
Published: Jun 1, 2018
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