Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Extraction-based blood coagulation activity of marigold leaf: a comparative study

Extraction-based blood coagulation activity of marigold leaf: a comparative study Marigold (Tagetes erecta), besides being an ornamental plant, has various medicinal properties—it is nematocidal, fungicidal, antibacterial and insecticidal and aids in wound healing. Our work is focused on the blood clotting activity of its leaf extracts. Extraction was done by conventional as well as the Soxhlet method, which was found to be much more efficient using a 1:1 ratio of ethanol to water as solvent. Blood clotting activity of the leaf extract was examined using prothrombin time test using the Owren method. For both extraction methods, the yield percentage and coagulation activity in terms of coagulation time were analysed. Marigold leaf extract obtained using the Soxhlet method has shown very good blood coagulation properties in lower quantities—in the range of microlitres. Further research is needed for identification and quantification of its bioactive compounds, which could be purified further and encapsulated. Since marigold leaf has antibacterial properties too, therefore, it might be possible in the future to develop an antiseptic with blood coagulation activity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Extraction-based blood coagulation activity of marigold leaf: a comparative study

Download PDF
4 pages

Loading next page...
 
/lp/springer-journals/extraction-based-blood-coagulation-activity-of-marigold-leaf-a-siyJxCQXAE
Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer-Verlag London
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1618-565X
DOI
10.1007/s00580-014-1943-5
Publisher site
See Article on Publisher Site

Abstract

Marigold (Tagetes erecta), besides being an ornamental plant, has various medicinal properties—it is nematocidal, fungicidal, antibacterial and insecticidal and aids in wound healing. Our work is focused on the blood clotting activity of its leaf extracts. Extraction was done by conventional as well as the Soxhlet method, which was found to be much more efficient using a 1:1 ratio of ethanol to water as solvent. Blood clotting activity of the leaf extract was examined using prothrombin time test using the Owren method. For both extraction methods, the yield percentage and coagulation activity in terms of coagulation time were analysed. Marigold leaf extract obtained using the Soxhlet method has shown very good blood coagulation properties in lower quantities—in the range of microlitres. Further research is needed for identification and quantification of its bioactive compounds, which could be purified further and encapsulated. Since marigold leaf has antibacterial properties too, therefore, it might be possible in the future to develop an antiseptic with blood coagulation activity.

Journal

Comparative Clinical Pathology – Springer Journals

Published: Jun 8, 2014

References

  • Studies on the antioxidant and analgesic activities of Aztec marigold (Tagetes erecta) flowers
    Bashir, S; Gilani, AH
  • Antioxidant properties of marigold extracts
    Ćetković, GS; Djilas, SM; Čanadanović-Brunet, JM; Tumbas, VT
  • Practical haematology
    Dacie, JV; Lewis, SM
  • Processing of Rosa rubiginosa: extraction of oil and antioxidant substances
    Franco, D; Pinelo, M; Sineiro, J; Nunez, MJ
  • Samples: from the patient to the laboratory, list of analytes preanalytical variables
    Guder, WG; Narayanan, S; Wisser, H; Zawta, B
  • EDTA samples are stable for prothrombin time measurement by combined thromboplastin reagent
    Horsti, J
  • Has the Quick or the Owren prothrombin time method the advantage in harmonization for the International Normalized Ratio system?
    Horsti, J
  • Recent extraction techniques for natural products: microwave-assisted extraction and pressurized solvent extraction
    Kaufmann, B; Christen, P
  • Comparison of microwave-assisted hydrodistillation and hydrodistillation methods for the analysis of volatile secondary metabolites
    Kosar, M; Özek, T; Göger, F; Kürkcüoglu, M; Baser, KHC
  • Effects of solvent properties on the Soxhlet extraction of diterpenoid lactones from Andrographis paniculata leaves
    Kumoro, AC; Hasan, M; Singh, H
  • Clinical laboratory medicine: clinical application of laboratory data
    Ravel, R
  • Clinical guide to laboratory test
    Tietz, NW
  • Tagetes: a multipurpose plant
    Vasudevan, P; Kashyup, S; Sharma, S