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Extracellular Proteolytic Enzymes of Azospirillum brasilense Strain Sp7 and Regulation of Their Activity by a Homologous Lectin

Extracellular Proteolytic Enzymes of Azospirillum brasilense Strain Sp7 and Regulation of Their... It was found that Azospirillum brasilense strain Sp7 is able to produce extracellular proteolytic enzymes. The enzymes were active within a broad range of pH values, with two peaks of activity being located in the acid and alkaline pH areas; required calcium ions; and exhibited substrate specificity with respect to azogelatin. Zymography allowed at least four proteolytic enzymes with molecular weights of 32, 45, 52, and 174 kDa to be detected in A. brasilense Sp7 culture liquid. It was shown that the lectin from A. brasilense Sp7 can inhibit proteolytic enzymes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Extracellular Proteolytic Enzymes of Azospirillum brasilense Strain Sp7 and Regulation of Their Activity by a Homologous Lectin

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References (15)

Publisher
Springer Journals
Copyright
Copyright © 2005 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Medical Microbiology; Biochemistry, general; Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1007/s10438-005-0066-9
Publisher site
See Article on Publisher Site

Abstract

It was found that Azospirillum brasilense strain Sp7 is able to produce extracellular proteolytic enzymes. The enzymes were active within a broad range of pH values, with two peaks of activity being located in the acid and alkaline pH areas; required calcium ions; and exhibited substrate specificity with respect to azogelatin. Zymography allowed at least four proteolytic enzymes with molecular weights of 32, 45, 52, and 174 kDa to be detected in A. brasilense Sp7 culture liquid. It was shown that the lectin from A. brasilense Sp7 can inhibit proteolytic enzymes.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Jul 12, 2005

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