Access the full text.
Sign up today, get DeepDyve free for 14 days.
Revathi Masilamani, O. Sharma, S. Muthuvel, S. Natarajan (2013)
Cloning, expression of b-1,3-1,4 glucanase from Bacillus subtilis SU40 and the effect of calcium ion on the stability of recombinant enzyme: in vitro and in silico analysisBioinformation, 9
T. Wen, Jui-Lin Chen, Shu-hua Lee, N. Yang, L. Shyur (2005)
A truncated Fibrobacter succinogenes 1,3-1,4-beta-d-glucanase with improved enzymatic activity and thermotolerance.Biochemistry, 44 25
M. Bang, I. Villadsen, T. Sandal (1999)
Cloning and characterization of an endo-β-1,3(4)glucanase and an aspartic protease from Phaffia rhodozyma CBS 6938Applied Microbiology and Biotechnology, 51
M Ekinci, S. McCrae, H Flint (1997)
Isolation and overexpression of a gene encoding an extracellular beta-(1,3-1,4)-glucanase from Streptococcus bovis JB1Applied and Environmental Microbiology, 63
Marilyn Anderson, Bruce Stone (1975)
A new substrate for investigating the specificity of β‐glucan hydrolasesFEBS Letters, 52
Huo-qing Huang, Peilong Yang, Huiying Luo, H. Tang, Na Shao, T. Yuan, Yaru Wang, Yingguo Bai, B. Yao (2008)
High-level expression of a truncated 1,3-1,4-β-d-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentationApplied Microbiology and Biotechnology, 78
T. McCarthy, O. Hanniffy, A. Savage, M. Tuohy (2003)
Catalytic properties and mode of action of three endo-beta-glucanases from Talaromyces emersonii on soluble beta-1,4- and beta-1,3;1,4-linked glucans.International journal of biological macromolecules, 33 1-3
Yingguo Bai, Jianshe Wang, Zhifang Zhang, P. Shi, Huiying Luo, Huo-qing Huang, Chunliang Luo, B. Yao (2010)
A novel family 9 β-1,3(4)-glucanase from thermoacidophilic Alicyclobacillus sp. A4 with potential applications in the brewing industryApplied Microbiology and Biotechnology, 87
K. Welfle, O. Politz, R. Borriss, R. Misselwitz, H. Welfle (1996)
Individual amino acids in the N‐terminal loop region determine the thermostability and unfolding characteristics of bacterial glucanasesProtein Science, 5
W. Vahjen, O. Simon (1999)
Biochemical characteristics of non starch polysaccharide hydrolyzing enzyme preparations designed as feed additives for poultry and piglet nutrition.Archiv fur Tierernahrung, 52 1
M. Choct, M. Bedford, G. Partridge (2000)
Enzyme supplementation of poultry diets based on viscous cereals.
M. Hahn, O. Olsen, O. Politz, R. Borriss, U. Heinemann (1995)
Crystal Structure and Site-directed Mutagenesis of Bacillus macerans Endo-1,31,4--glucanase (*)The Journal of Biological Chemistry, 270
D. Teng, Ying Fan, Yalin Yang, Z. Tian, Jin Luo, Jian-hua Wang (2007)
Codon optimization of Bacillus licheniformis β-1,3-1,4-glucanase gene and its expression in Pichia pastorisApplied Microbiology and Biotechnology, 74
J. Cereghino, J. Cregg (2000)
Heterologous protein expression in the methylotrophic yeast Pichia pastoris.FEMS microbiology reviews, 24 1
O. Gaiser, K. Piotukh, M. Ponnuswamy, A. Planas, R. Borriss, U. Heinemann (2006)
Structural basis for the substrate specificity of a Bacillus 1,3-1,4-β-glucanaseJournal of Molecular Biology, 357
A. Boyce, G. Walsh (2007)
Production, purification and application-relevant characterisation of an endo-1,3(4)-β-glucanase from Rhizomucor mieheiApplied Microbiology and Biotechnology, 76
M. Almirall, E. Esteve-Garcia (1995)
In vitro stability of a β-glucanase preparation from Trichoderma longibrachiatum and its effect in a barley based diet fed to broiler chicksAnimal Feed Science and Technology, 54
R. Kawai, K. Igarashi, Makoto Yoshida, M. Kitaoka, M. Samejima (2005)
Hydrolysis of β-1,3/1,6-glucan by glycoside hydrolase family 16 endo-1,3(4)-β-glucanase from the basidiomycete Phanerochaete chrysosporiumApplied Microbiology and Biotechnology, 71
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
G. Furtado, L. Ribeiro, Camila Santos, C. Tonoli, Angelica Souza, Renata Oliveira, M. Murakami, R. Ward (2011)
Biochemical and structural characterization of a Beta-1,3 1,4-glucanase from Bacillus subtilis 168Process Biochemistry, 46
Ana-Belén Martín-Cuadrado, Javier Dedo, María Medina-Redondo, T. Fontaine, F. Rey, J. Latgé, C. Aldana (2008)
The Schizosaccharomyces pombe endo‐1,3‐β‐glucanase Eng1 contains a novel carbohydrate binding module required for septum localizationMolecular Microbiology, 69
(2001)
Community Register of Feed Additives Pursuant to Regulation ( EC ) No . 1831 / 2003 , Appendixes 3 and 4 , Annex : List of Additives
R. Borriss, K. Buettner, Pekka Maentsaelae (1990)
Structure of the beta- 1,3-1,4-glucanase gene ofBacillus macerans: Homologies to other beta-glucanasesMolecular and General Genetics MGG, 222
M. Wolf, A. Géczi, O. Simon, R. Borriss (1995)
Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase.Microbiology, 141 ( Pt 2)
D. Teng, Jian-hua Wang, Ying Fan, Yalin Yang, Z. Tian, Jin Luo, Guanpin Yang, Fan Zhang (2006)
Cloning of β-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039 (CGMCC 0635) and its expression in Escherichia coli BL21 (DE3)Applied Microbiology and Biotechnology, 72
M. Martínez, F. Reyes, R. Lahoz, M. Pérez-Leblic (1983)
Lytic enzymes in autolysis of Botrytis cinereaFems Microbiology Letters, 19
G. Miller (1959)
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing SugarAnalytical Chemistry, 31
The isolation, heterologous expression, and characterization of a new, thermostable β-glucanase from Paenibacillus jamilae are described. The bgl26 gene from the P. jamilae Bg1 VKPM B-13 093 strain, which consists of 714 nucleotides, encodes endo-1,3-1,4-β-glucanase (EC 3.2.1.73), which contains 213 amino acids and 24 residues of the putative signal peptide in the N-terminal region. The nucleotide sequence of the bgl26 gene and the amino acid sequence of the mature Bgl26 protein have the greatest homology with the sequences of the Paenibacillus macerans endo-1,3-1,4-β-glucanase (82 and 88%, respectively). A gene fragment encoding the mature protein was expressed in Pichia pastoris. The purified recombinant enzyme Bgl26 was active against barley β-glucan. The optimal pH for the enzyme activity was 7.0, and the optimum temperature range was 40–45°C. The specific β-glucanase activity was at the level of 6650 U/mg of protein; KM and Vmax were equal to 6.4 ± 0.3 mg/mL and 9450.1 ± 471.2 μmol/(min mg), respectively. The recombinant protein Bgl26 was characterized by a high pH and thermal stability, as well as resistance to digestive enzymes. It was also shown that Co2+ ions have a positive effect on enzyme activity.
Applied Biochemistry and Microbiology – Springer Journals
Published: Dec 1, 2020
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.