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Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of Industrially Important Enzyme Properties

Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 9, pp. 877–881. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 1, pp. 38–44. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of Industrially Important Enzyme Properties a, a a a M. G. Tarutina *, M. N. Lazareva , E. I. Semenko , and S. P. Sineoky State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (GosNIIgenetika, Kurchatov Institute NRC), Moscow, 117545 Russia *e-mail: m_tarutina@mail.ru Received November 8, 2018; revised November 28, 2018; accepted January 15, 2019 Abstract—Eff icient expression of the man1 gene for Aspergillus aculeatus β-1,4-mannanase was demonstrated in Pichia pastoris yeast for the f irst time, and industrially important characteristics of the synthesized enzyme were confirmed. β-Mannanases are industrial enzymes that can be used, in particular, in the feed industry. The most important requirements for feed enzymes are a broad pH range, thermal stability, and high specif ic activity. The obtained data indicate that the man1 gene from A. aculeatus is potentially useful for the construc- tion of industrial mannanase producers http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of Industrially Important Enzyme Properties

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Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819090084
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 9, pp. 877–881. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 1, pp. 38–44. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of Industrially Important Enzyme Properties a, a a a M. G. Tarutina *, M. N. Lazareva , E. I. Semenko , and S. P. Sineoky State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (GosNIIgenetika, Kurchatov Institute NRC), Moscow, 117545 Russia *e-mail: m_tarutina@mail.ru Received November 8, 2018; revised November 28, 2018; accepted January 15, 2019 Abstract—Eff icient expression of the man1 gene for Aspergillus aculeatus β-1,4-mannanase was demonstrated in Pichia pastoris yeast for the f irst time, and industrially important characteristics of the synthesized enzyme were confirmed. β-Mannanases are industrial enzymes that can be used, in particular, in the feed industry. The most important requirements for feed enzymes are a broad pH range, thermal stability, and high specif ic activity. The obtained data indicate that the man1 gene from A. aculeatus is potentially useful for the construc- tion of industrial mannanase producers

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Dec 5, 2019

References