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Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of Industrially Important Enzyme Properties

Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 9, pp. 877–881. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 1, pp. 38–44. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of Industrially Important Enzyme Properties a, a a a M. G. Tarutina *, M. N. Lazareva , E. I. Semenko , and S. P. Sineoky State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (GosNIIgenetika, Kurchatov Institute NRC), Moscow, 117545 Russia *e-mail: m_tarutina@mail.ru Received November 8, 2018; revised November 28, 2018; accepted January 15, 2019 Abstract—Eff icient expression of the man1 gene for Aspergillus aculeatus β-1,4-mannanase was demonstrated in Pichia pastoris yeast for the f irst time, and industrially important characteristics of the synthesized enzyme were confirmed. β-Mannanases are industrial enzymes that can be used, in particular, in the feed industry. The most important requirements for feed enzymes are a broad pH range, thermal stability, and high specif ic activity. The obtained data indicate that the man1 gene from A. aculeatus is potentially useful for the construc- tion of industrial mannanase producers http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of Industrially Important Enzyme Properties

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Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819090084
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 9, pp. 877–881. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 1, pp. 38–44. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Expression of Aspergillus aculeatus β-Mannanase in Pichia pastoris Yeast and Analysis of Industrially Important Enzyme Properties a, a a a M. G. Tarutina *, M. N. Lazareva , E. I. Semenko , and S. P. Sineoky State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (GosNIIgenetika, Kurchatov Institute NRC), Moscow, 117545 Russia *e-mail: m_tarutina@mail.ru Received November 8, 2018; revised November 28, 2018; accepted January 15, 2019 Abstract—Eff icient expression of the man1 gene for Aspergillus aculeatus β-1,4-mannanase was demonstrated in Pichia pastoris yeast for the f irst time, and industrially important characteristics of the synthesized enzyme were confirmed. β-Mannanases are industrial enzymes that can be used, in particular, in the feed industry. The most important requirements for feed enzymes are a broad pH range, thermal stability, and high specif ic activity. The obtained data indicate that the man1 gene from A. aculeatus is potentially useful for the construc- tion of industrial mannanase producers

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Dec 5, 2019

References

  • Characterization of galactomannan derivatives in roasted coffee beverages
    Nunes, F.M.; Reis, A.; Domingues, M.R.; Coimbra, M.A.
  • An overview of mannan structure and mannan-degrading enzyme systems
    Moreira, L.R.; Filho, E.X.
  • Hemicellulose and Hemicellulases
    Puls, J.; Schuseil, J.
  • New families in the classification of glycosyl hydrolases based on amino-acid sequence similarities
    Henrissat, B.; Bairoch, A.
  • The carbohydrate-active EnZymes database (CAZy): an expert resource for glycogenomics
    Cantarel, B.L.; Coutinho, P.M.; Rancurel, C.; Bernard, T.; Lombard, V.; Henrissat, B.
  • Expression of the Aspergillus aculeatus endo-beta-1,4-mannanase encoding gene (man1) in Saccharomyces cerevisiae and characterization of the recombinant enzyme
    Hahn-Hagerdal, B.; Stalbrand, H.
  • Expression cloning, purification and characterization of a beta-1,4-mannanase from Aspergillus aculeatus
    Christgau, S.; Kauppinen, S.; Vind, J.; Kofod, L.V.; Dalboge, H.
  • Production of the Aspergillus aculeatus endo-1,4-mannanase in A. niger
    van Zyl, P.J.; Moodley, V.; Rose, S.H.; Roth, R.L.; van Zyl, W.H.
  • Expression and evaluation of enzymes required for the hydrolysis of galactomannan
    Malherbe, A.R.; Rose, S.H.; Viljoen-Bloom, M.; van Zyl, W.H.
  • Heterologous expression and optimized production of an Aspergillus aculeatus endo-1,4-β-mannanase in Yarrowia lipolytica
    Roth, R.; Moodley, V.; van Zyl, P.
  • Modeling of growth and energy metabolism of Pichia pastoris producing a fusion protein
    Yanic, M.; Rotticci-Mulder, M.; Martinelle, M.
  • High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts
    Hasslacher, M.; Schall, M.; Hayn, M.; Bona, R.; Rumbold, K.; Luckl, J.; Griengl, H.; Kohlwein, S.D.; Schwab, H.
  • Overexpression of an optimized Aspergillus sulphureus β-mannanase gene in Pichia pastoris
    Chen, X.; Qiao, J.; Yu, H.; Cao, Y.