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Expression and Characterization of Phytase from Obesumbacterium proteus in Pichia pastoris

Expression and Characterization of Phytase from Obesumbacterium proteus in Pichia pastoris ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 7, pp. 741–747. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2018, published in Biotekhnologiya, 2018, Vol. 34, No. 4, pp. 18–25. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Expression and Characterization of Phytase from Obesumbacterium proteus in Pichia pastoris a, a a a T. L. Gordeeva *, L. N. Borshchevskaya , A. N. Kalinina , S. P. Sineoky , b b S. P. Voronin , and M. D. Kashirskaya State Research Institute for Genetics and Selection of Industrial Microorganisms of the National Research Center, Kurchatov Institute (GOSNIIGENETIKA, NRC, Kurchatov Institute), Moscow, 117545 Russia Bioamide Joint Venture, Saratov, 410033 Russia *e-mail: tatiana.gordeeva@mail.ru Received May 24, 2018; revised May 24, 2018; accepted July 6, 2018 Abstract—In order to increase the expression level of gene for recombinant phytase in Pichia pastoris, a syn- thetic sequence of the phyOp-mod gene, which encodes phytase from Obesumbacterium proteus, has been designed and optimized by its codon composition. The GC content was adjusted to 48.33%, and AT-rich seg- ments were eliminated. The main characteristics for the purif ied enzyme are established. The optimal pH and temperature values for РhyOp phytase functioning prove to be http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Expression and Characterization of Phytase from Obesumbacterium proteus in Pichia pastoris

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References (39)

Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819070032
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 7, pp. 741–747. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2018, published in Biotekhnologiya, 2018, Vol. 34, No. 4, pp. 18–25. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Expression and Characterization of Phytase from Obesumbacterium proteus in Pichia pastoris a, a a a T. L. Gordeeva *, L. N. Borshchevskaya , A. N. Kalinina , S. P. Sineoky , b b S. P. Voronin , and M. D. Kashirskaya State Research Institute for Genetics and Selection of Industrial Microorganisms of the National Research Center, Kurchatov Institute (GOSNIIGENETIKA, NRC, Kurchatov Institute), Moscow, 117545 Russia Bioamide Joint Venture, Saratov, 410033 Russia *e-mail: tatiana.gordeeva@mail.ru Received May 24, 2018; revised May 24, 2018; accepted July 6, 2018 Abstract—In order to increase the expression level of gene for recombinant phytase in Pichia pastoris, a syn- thetic sequence of the phyOp-mod gene, which encodes phytase from Obesumbacterium proteus, has been designed and optimized by its codon composition. The GC content was adjusted to 48.33%, and AT-rich seg- ments were eliminated. The main characteristics for the purif ied enzyme are established. The optimal pH and temperature values for РhyOp phytase functioning prove to be

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Applied Biochemistry and Microbiology – Springer Journals

Published: Dec 5, 2019

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