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Evaluation of the trisodium salt of 3-phosphoglycerate as a fuel for red cell storage

Evaluation of the trisodium salt of 3-phosphoglycerate as a fuel for red cell storage During red cell storage the pH of the medium and that of the intracellular fluid decreases. The decrease in pH is due to glycolysis and is a likely contributor to red cell storage lesion. The trisodium salt of 3-phosphoglycerate (PGA) would be an ideal non-acid producing fuel for stored red cells as it would enter the glycolytic pathway below the acid-producing steps (glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase), and in comparison with glucose, would enable the production of equivalent amounts of ATP per carbon of the fuel. A medium containing PGA (PGA) was evaluated for 21 days in comparison with a standard dextrose-phosphate-citrate additive solution (DCP) and a medium containing minimal fuel (NF). Fuel utilisation, lactate and pyruvate production, pH, adenylates, Hb, GSH, Na+ and K+ were measured, and in vitro incubations were used to assess the potential for flux through glycolysis and the pentose pathway. DCP was the superior medium, although this is not evident for the first 7 days. 3-Phosphoglycerate did enter the cells, but was metabolised too slowly to be a useful fuel. The cells in PGA were similar to those in DCP in one respect in that they maintained their pentose pathway potential. Apart from flux through the pentose pathway, PGA and NF were identical in that all tests indicated substantial storage lesion. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Evaluation of the trisodium salt of 3-phosphoglycerate as a fuel for red cell storage

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References (34)

Publisher
Springer Journals
Copyright
Copyright © 1992 by Springer-Verlag
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1433-2973
DOI
10.1007/BF02984525
Publisher site
See Article on Publisher Site

Abstract

During red cell storage the pH of the medium and that of the intracellular fluid decreases. The decrease in pH is due to glycolysis and is a likely contributor to red cell storage lesion. The trisodium salt of 3-phosphoglycerate (PGA) would be an ideal non-acid producing fuel for stored red cells as it would enter the glycolytic pathway below the acid-producing steps (glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase), and in comparison with glucose, would enable the production of equivalent amounts of ATP per carbon of the fuel. A medium containing PGA (PGA) was evaluated for 21 days in comparison with a standard dextrose-phosphate-citrate additive solution (DCP) and a medium containing minimal fuel (NF). Fuel utilisation, lactate and pyruvate production, pH, adenylates, Hb, GSH, Na+ and K+ were measured, and in vitro incubations were used to assess the potential for flux through glycolysis and the pentose pathway. DCP was the superior medium, although this is not evident for the first 7 days. 3-Phosphoglycerate did enter the cells, but was metabolised too slowly to be a useful fuel. The cells in PGA were similar to those in DCP in one respect in that they maintained their pentose pathway potential. Apart from flux through the pentose pathway, PGA and NF were identical in that all tests indicated substantial storage lesion.

Journal

Comparative Clinical Pathology – Springer Journals

Published: Nov 5, 2008

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