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V Morton, J Jean, J Farber, K Mattison (2009)
Detection of noroviruses in readyto-eat foods by using carbohydrate-coated magnetic beadsApplied and Environmental Microbiology, 75
E Scallan, RM Hoekstra, FJ Angulo, RV Tauxe, MA Widdowson, SL Roy, JL Jones, PM Griffin (2011)
Foodborne illness acquired in the United States major pathogensEmerging Infectious Diseases, 17
GH Fleet, P Heiskanen, I Reid, KA Buckle (2000)
Foodborne viral illness status in AustraliaInternational Journal of Food Microbiology, 59
JB Gentry, J Vinje, EK Lipp (2009)
A rapid and efficient method for quantitation of genogroups I and II norovirus from oysters and application in other complex environmental samplesJournal of Virological Methods, 156
L Pan, Q Zhang, X Li, P Tian (2012)
Detection of human norovirus in cherry tomatoes, blueberries and vegetable salad by using a receptor-binding capture and magnetic sequestration (RBCMS) methodFood Microbiology, 30
J Meng, S Zhao, MP Doyle, SE Mitchell, S Kresovich (1996)
Polymerase chain reaction for detecting Escherichia coli O157:H7International Journal of Food Microbiology, 32
KB Lee, H Lee, SD Ha, DS Cheon, C Choi (2012)
Comparative analysis of viral concentration 354 methods for detecting the HAV genome using real-time RT-PCR amplificationFood and Environmental Virology, 355
K Scherer, R Johne, C Schrader, L Ellerbroek, J Schulenburg, G Klein (2010)
Comparison of two extraction methods for viruses in food and application in a norovirus gastroenteritis outbreakJournal of Virological Methods, 169
ML Halliday, LY Kang, TK Zhou, MD Hu, QC Pan, TY Fu, YS Huang, SL Hu (1991)
An epidemic of hepatitis A attributable to the ingestion of raw clams in Shanghai, ChinaJournal of Infectious Diseases, 164
DH Kingsley, GP Richards (2001)
Rapid and efficient extraction method for reverse transcription-PCR detection of hepatitis A and norwalk-like viruses in shellfishApplied and Environmental Microbiology, 67
L Baert, M Uyttendaele, J Debevere (2008)
Evaluation of viral extraction methods on a broad range of ready-to-eat foods with conventional and realtime RT-PCR for norovirus GII detectionInternational Journal of Food Microbiology, 123
L Bakkali Kassimi, M Gonzague, A Boutrouille, C Cruciere (2002)
Detection of Encephalomyocarditis virus in clinical samples by immunomagnetic separation and one-step RT-PCRJournal of Virological Methods, 101
M Koopmans, E Duizer (2004)
Foodborne viruses: An emerging problemInternational Journal of Food Microbiology, 90
SY Lee, KI Jang, GJ Woo, HS Kwak, KY Kim (2007)
Development of protocol for the effective detection of feline calicivirus as norovirus surrogate in oyster and lettuceKorean Journal of Food Science and Technology, 39
TM Fumian, JPG Leite, VA Marin, MP Miagostovich (2009)
A rapid procedure for detecting noroviruses from cheese and fresh lettuceJournal of Virological Methods, 155
LP Mansfield, SJ Forsythe (1993)
Immunomagnetic separation as an alternative to enrichment broths for Salmonella detectionLetters in Applied Microbiology, 16
DE Berg, MA Kohn, TA Farley, LM McFarland (2000)
Multi–state outbreaks of acute gastroenteritis traced to fecal–contaminated oysters harvested in LouisianaJournal of Infectious Diseases, 181
C Moore, EM Clark, CI Gallimore, SA Corden, JJ Gray, D Westmoreland (2004)
Evaluation of a broadly reactive nucleic acid sequence based amplification assay for the detection of noroviruses in faecal materialJournal of Clinical Virology, 29
G Flekna, W Schneeweiss, FJM Smulders, M Wagner, I Hein (2007)
Real-time PCR method with statistical analysis to compare the potential of DNA isolation methods to remove PCR inhibitors from samples for diagnostic PCRMolecular and Cellular Probes, 21
IG Wilson (1997)
Inhibition and facilitation of nucleic acid amplificationApplied and Environmental Microbiology, 63
TK Graczyk, KJ Schwab (2000)
Foodborne infections vectored by molluscan shellfishCurrent gastroenterology reports, 2
G Sánchez, A Bosch, RM Pintó (2007)
Hepatitis A virus detection in food: current and future prospectsLetters in Applied Microbiology, 45
L Kittigul, K Pombubpa, S Sukonthalux, T Rattanatham, F Utrarachkij (2011)
Noroviruses in oysters from local markets and oyster farms in southern ThailandSoutheast Asian Journal of Tropical Medicine and Public Health, 42
NP Rijpens, LMF Herman (2002)
Molecular methods for identification and detection of bacterial food pathogensJournal of AOAC International, 85
SF Dowell, C Groves, KB Kirkland, HG Cicirello, T Ando, Q Jin, JR Gentsch, SS Monroe, CD Humphrey, C Slemp (1995)
A multistate outbreak of oyster associated gastroenteritis: Implications for interstate tracing of contaminated shellfishJournal of Infectious Diseases, 171
DJ Coleman, KE Chick, KJ Nye (1995)
An evaluation of immunomagnetic separation for the detection of salmonellas in raw chicken carcassesLetters in Applied Microbiology, 21
AC Espinosa, M Mazari-Hiriart, R Espinosa, L Maruri-Avidal, E Mendez, CF Arias (2008)
Infectivity and genome persistence of rotavirus and astrovirus in roundwater and surface waterWater Research, 42
BF Christensen, D Lees, K Henshilwood, T Bjergskov, J Green (1998)
Human enteric viruses in oysters causing a large outbreak of human food borne infection in 1996–1997Journal of Shellfish Research, 17
SW Kim, SB Baek, JH Ha, MH Lee, CS Choi, SD Ha (2012)
Chlorine treatment to inactivate norovirus on food contact surfacesJournal of Food Protection, 75
AC Schultz, P Saadbye, J Hoorfar, B Nørrung (2007)
Comparison of methods for detection of norovirus in oystersInternational Journal of Food Microbiology, 114
J Wang, Z Deng (2012)
Detection and forecasting of oyster norovirus outbreaks: Recent advances and future perspectivesMarine Environmental Research, 80
PA Chapman, DJ Wright, CA Siddons (1994)
A comparison of immunomagnetic separation and direct culture for the isolation of verocytotoxin-producing Escherichia coli O157 from bovine faecesJournal of Medical Microbiology, 40
BE Moore (1993)
Survival of human-immunodeficiency-virus (HIV), HIV-infected lymphocytes, and poliovirus in waterApplied and Environmental Microbiology, 59
R Morales-Rayas, PFG Wolffs, MW Griffiths (2009)
Anion-exchange filtration and real-time PCR for the detection of a norovirus surrogate in foodJournal of Food Protection, 72
UD Parashar, SS Monroe (2001)
“Norwalk-like viruses” as a cause of foodborne disease outbreaksReviews in Medical Virology, 11
M Uyttendaele, I Hoorde, J Debevere (2000)
The use of immuno-magnetic separation (IMS) as a tool in a sample preparation method for direct detection of L. monocytogenes in cheeseInternational Journal of Food Microbiology, 54
YB Park, Y-H Cho, YM Jee, GP Ko (2008)
Immunomagnetic separation combined with real-time reverse transcriptase PCR assays for detection of norovirus in contaminated foodApplied and Environmental Microbiology, 74
PS Mead, L Slutsker, V Dietz, LF McCaig, JS Bresee, C Shapiro, PM Griffin, RV Tauxe (1999)
Food-related illness and death in the United StatesEmerging Infectious Diseases, 5
G Simmons, G Greening, W Gao, D Campbell (2001)
Raw oyster consumption and outbreaks of viral gastroenteritis in New Zealand: evidence for risk to the public's health. Aust. N.Z.JPublic Health, 25
S Cheong, C Lee, WC Choi, CH Lee, SJ Kim (2009)
Concentration method for the detection of enteric viruses from large volumes of foodsJournal of Food Protection, 72
Outbreaks of viral diseases are frequently associated with the consumption of minimally processed shellfish. Among the viruses in these outbreaks, hepatitis A virus (HAV) and human norovirus (NoV) have been increasingly reported as the most common food-borne pathogens. These viruses must be concentrated in tested samples in order to be detected. In this study, a method for the detection of NoV and HAV in shellfish using an immuno-magnetic separation (IMS) procedure combined with reverse transcriptase (RT)-PCR was developed. The IMS/RT-PCR method was applied to investigate the recovery rates of HAV, NoV GI.1, and GII.4 from oyster and mussel. Based on IMS/RT-PCR results, recovery rates for HAV from oyster and mussel test samples were 2.4 and 1.1 %, respectively. The NoV GI.1 recovery rates from oyster and mussel samples were 4.9–9.2 % (mean 6.9 %) and 4.3–8.6 % (mean 6.2 %), respectively, and the NoV GII.4 recovery rates were 8.8 and 8.5 %, respectively. These results verified that HAV, NoV GI.1, and GII.4 can be detected in all the test samples using the IMS/RT-PCR method, although the three inoculated viruses were recovered with low efficiency. In conclusion, the IMS/RT-PCR method can be used to efficiently and rapidly detect viruses such as HAV and NoV in shellfish such as oyster and mussel.
Food and Environmental Virology – Springer Journals
Published: Jun 22, 2014
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