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Eosinophil Induction in Chicken Embryos and its Responsiveness to the Chemoattractant fMLP

Eosinophil Induction in Chicken Embryos and its Responsiveness to the Chemoattractant fMLP Inoculation of concanavalin-A (Con-A) into the allantoic cavity of chicken embryos resulted in a massive infiltration of eosinophilic granulocytes into the spleens. Immunohistochemical staining of formalin-fixed, paraffin-embedded spleen sections with monoclonal anti-major basic protein (MBP) serum revealed that these cells contain MBP, and were therefore eosinophils. Transmission electron microscopic examination of the spleens showed that these cells contained bi-lobulated nuclei and many granules of various sizes and shapes. These granules, however, did not contain a central core, which is a prominent feature in their mammalian counterparts. The eosinophils isolated from adult chickens underwent remarkable surface changes when incubated with the chemoattractant formyl-methionyl-leucyl-phenylalanine (fMLP) by forming and extending surface projections. Adult eosinophils were immotile, but showed chemotactic response when fMLP was used as a chemoattractant in chemotaxis assays. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Eosinophil Induction in Chicken Embryos and its Responsiveness to the Chemoattractant fMLP

LAM, K. M.; MUNN, R. J.
Comparative Clinical Pathology , Volume 11 (3) – Jul 1, 2002
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References (11)

Publisher
Springer Journals
Copyright
Copyright © 2002 by Springer-Verlag London Limited
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1618-565X
DOI
10.1007/s005800200015
Publisher site
See Article on Publisher Site

Abstract

Inoculation of concanavalin-A (Con-A) into the allantoic cavity of chicken embryos resulted in a massive infiltration of eosinophilic granulocytes into the spleens. Immunohistochemical staining of formalin-fixed, paraffin-embedded spleen sections with monoclonal anti-major basic protein (MBP) serum revealed that these cells contain MBP, and were therefore eosinophils. Transmission electron microscopic examination of the spleens showed that these cells contained bi-lobulated nuclei and many granules of various sizes and shapes. These granules, however, did not contain a central core, which is a prominent feature in their mammalian counterparts. The eosinophils isolated from adult chickens underwent remarkable surface changes when incubated with the chemoattractant formyl-methionyl-leucyl-phenylalanine (fMLP) by forming and extending surface projections. Adult eosinophils were immotile, but showed chemotactic response when fMLP was used as a chemoattractant in chemotaxis assays.

Journal

Comparative Clinical Pathology – Springer Journals

Published: Jul 1, 2002

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