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Enzymatic kinetic resolution in flow for chiral mandelic acids

Enzymatic kinetic resolution in flow for chiral mandelic acids During the kinetic resolution process, increased substrate conversion often compromised the optical purity of products. A strategy integrated biotechnology and microtechnology was developed to kinetic resolution of mandelic acids. The effects of temperature, pH, solvent, reaction time, flow rate on the hydrolysis were conducted. Under optimal conditions, the MCR reaction afforded 95% eep, whereas the batch reaction yielded 65% eep. The kinetics of hydrolysis was also investigated. The value for Km and Vmax in batch was 12 mM and 11 mM s−1, while that in MCR was 8 mM, 45 mM s−1. This means more substrates can be transferred to the active site on the enzyme in MCR at the same concentration. This is a benefit from the excellent mass transfer limitations in MCR. Furthermore, the apparent Vmax was significantly increased, closer to the intrinsic rate of reaction. Finally, the strong mass transfer in MCR leads to an expanded rate difference between (R) enantiomers and (S) enantiomers, improving the eep and E-values. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Flow Chemistry Springer Journals

Enzymatic kinetic resolution in flow for chiral mandelic acids

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Publisher
Springer Journals
Copyright
Copyright © Akadémiai Kiadó 2022
ISSN
2062-249X
eISSN
2063-0212
DOI
10.1007/s41981-022-00219-z
Publisher site
See Article on Publisher Site

Abstract

During the kinetic resolution process, increased substrate conversion often compromised the optical purity of products. A strategy integrated biotechnology and microtechnology was developed to kinetic resolution of mandelic acids. The effects of temperature, pH, solvent, reaction time, flow rate on the hydrolysis were conducted. Under optimal conditions, the MCR reaction afforded 95% eep, whereas the batch reaction yielded 65% eep. The kinetics of hydrolysis was also investigated. The value for Km and Vmax in batch was 12 mM and 11 mM s−1, while that in MCR was 8 mM, 45 mM s−1. This means more substrates can be transferred to the active site on the enzyme in MCR at the same concentration. This is a benefit from the excellent mass transfer limitations in MCR. Furthermore, the apparent Vmax was significantly increased, closer to the intrinsic rate of reaction. Finally, the strong mass transfer in MCR leads to an expanded rate difference between (R) enantiomers and (S) enantiomers, improving the eep and E-values.

Journal

Journal of Flow ChemistrySpringer Journals

Published: Jun 1, 2022

Keywords: Kinetic resolution; Lipase; Micro-reactor; Hydrolysis

References