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Enhanced amplification of bacterial and fungal DNA using a new type of DNA polymerase

Enhanced amplification of bacterial and fungal DNA using a new type of DNA polymerase In this study we compared the efficiency of direct colony PCR of bacterial and fungal DNA with Taq DNA polymerase and MightyAmp DNA Polymerase. The results show that whilst only 44.0 % of bacterial species representing 13 genera and 11.1 % of fungal species representing 18 genera could be amplified by DCPCR with Taq DNA polymerase, all 25 bacterial species and 27 fungal species including 1 biotrophic fungus Puccinia cannacearum could be amplified by DCPCR with MightyAmp DNA polymerase. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

Enhanced amplification of bacterial and fungal DNA using a new type of DNA polymerase

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References (13)

Publisher
Springer Journals
Copyright
Copyright © 2012 by Australasian Plant Pathology Society Inc.
Subject
Life Sciences; Agriculture; Plant Sciences; Ecology; Plant Pathology; Entomology
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1007/s13313-012-0144-4
Publisher site
See Article on Publisher Site

Abstract

In this study we compared the efficiency of direct colony PCR of bacterial and fungal DNA with Taq DNA polymerase and MightyAmp DNA Polymerase. The results show that whilst only 44.0 % of bacterial species representing 13 genera and 11.1 % of fungal species representing 18 genera could be amplified by DCPCR with Taq DNA polymerase, all 25 bacterial species and 27 fungal species including 1 biotrophic fungus Puccinia cannacearum could be amplified by DCPCR with MightyAmp DNA polymerase.

Journal

Australasian Plant PathologySpringer Journals

Published: Jun 24, 2012

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