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In this study we compared the efficiency of direct colony PCR of bacterial and fungal DNA with Taq DNA polymerase and MightyAmp DNA Polymerase. The results show that whilst only 44.0 % of bacterial species representing 13 genera and 11.1 % of fungal species representing 18 genera could be amplified by DCPCR with Taq DNA polymerase, all 25 bacterial species and 27 fungal species including 1 biotrophic fungus Puccinia cannacearum could be amplified by DCPCR with MightyAmp DNA polymerase.
Australasian Plant Pathology – Springer Journals
Published: Jun 24, 2012
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