Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Engineering Clostridium acetobutylicum for Enhanced Solvent Production by Overexpression of Pyruvate Decarboxylase from Zymomonas mobilis

Engineering Clostridium acetobutylicum for Enhanced Solvent Production by Overexpression of... Clostridium acetobutylicum DSM 792 has been characterized as a native acetone-butanol-ethanol (ABE) fermenting bacterium. C. acetobutylicm is used as a model organism to improve solvent production by metabolic engineering strategies. In this study, the ABE pathway in C. acetobutylicum was modified by overexpressing the pyruvate decarboxylase gene from Zymomonas mobilis under the control of 2 promoters, namely Pferr and Ppta (promoters of genes encoding pyruvate ferredoxin oxidoreductase and phosphotransacetylase) and the recombinant strains were designated as DSM 792(pPDC1) and DSM 792(pPDC2). The specific activity of the alcohol dehydrogenases in the pdc-expressing strains was higher than that in the wild-type strain. In batch fermentation experiments, the total ABE production from the recombinant strains DSM 792 (pPDC1) and DSM 792 (pPDC2) was 1.57 and 7.9 g/L, respectively, which was higher when compared to the wild type ABE production (0.64 g/L) under uncontrolled pH. The alcohol to acetone ratio (BE/A) was found to be 2.28, 2.77, and 5.26 in wild type, DSM 792 (pPDC1), and DSM 792 (pPDC2), respectively. This suggests that the re-routing of pyruvate by overexpression of the pdc could play a role in the generation of more reducing equivalents towards ethanol and butanol production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Engineering Clostridium acetobutylicum for Enhanced Solvent Production by Overexpression of Pyruvate Decarboxylase from Zymomonas mobilis

Loading next page...
 
/lp/springer-journals/engineering-clostridium-acetobutylicum-for-enhanced-solvent-production-0BC2uKnTLT
Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2021. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 5, pp. 611–617. © Pleiades Publishing, Inc., 2021.
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/s0003683821050045
Publisher site
See Article on Publisher Site

Abstract

Clostridium acetobutylicum DSM 792 has been characterized as a native acetone-butanol-ethanol (ABE) fermenting bacterium. C. acetobutylicm is used as a model organism to improve solvent production by metabolic engineering strategies. In this study, the ABE pathway in C. acetobutylicum was modified by overexpressing the pyruvate decarboxylase gene from Zymomonas mobilis under the control of 2 promoters, namely Pferr and Ppta (promoters of genes encoding pyruvate ferredoxin oxidoreductase and phosphotransacetylase) and the recombinant strains were designated as DSM 792(pPDC1) and DSM 792(pPDC2). The specific activity of the alcohol dehydrogenases in the pdc-expressing strains was higher than that in the wild-type strain. In batch fermentation experiments, the total ABE production from the recombinant strains DSM 792 (pPDC1) and DSM 792 (pPDC2) was 1.57 and 7.9 g/L, respectively, which was higher when compared to the wild type ABE production (0.64 g/L) under uncontrolled pH. The alcohol to acetone ratio (BE/A) was found to be 2.28, 2.77, and 5.26 in wild type, DSM 792 (pPDC1), and DSM 792 (pPDC2), respectively. This suggests that the re-routing of pyruvate by overexpression of the pdc could play a role in the generation of more reducing equivalents towards ethanol and butanol production.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Sep 1, 2021

Keywords: biofuels; acetone–butanol–ethanol fermentation; Clostridium acetobutylicum; metabolic engineering; pyruvate decarboxylase

References