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Electron Diffraction of Microcrystals on the Example of Lysozyme

Electron Diffraction of Microcrystals on the Example of Lysozyme Electron diffraction of microcrystals is a new powerful tool of cryo-electron microscopy, which makes it possible to determine the structure of three-dimensional crystals from a rotation series of diffraction patterns. Unlike X-ray crystallography, this method allows one to study micro- and even nanosized crystals. In this case, it is sufficient to have one or several crystals to obtain the structure with a high spatial resolution, which significantly reduces the necessary sample volume. The methodological aspects of application of electron diffraction of microcrystals are considered on the example of classical model protein: lysozyme. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Crystallography Reports Springer Journals

Electron Diffraction of Microcrystals on the Example of Lysozyme

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References (23)

Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2021. ISSN 1063-7745, Crystallography Reports, 2021, Vol. 66, No. 5, pp. 765–768. © Pleiades Publishing, Inc., 2021. Russian Text © The Author(s), 2021, published in Kristallografiya, 2021, Vol. 66, No. 5, pp. 738–741.
ISSN
1063-7745
eISSN
1562-689X
DOI
10.1134/s1063774521050084
Publisher site
See Article on Publisher Site

Abstract

Electron diffraction of microcrystals is a new powerful tool of cryo-electron microscopy, which makes it possible to determine the structure of three-dimensional crystals from a rotation series of diffraction patterns. Unlike X-ray crystallography, this method allows one to study micro- and even nanosized crystals. In this case, it is sufficient to have one or several crystals to obtain the structure with a high spatial resolution, which significantly reduces the necessary sample volume. The methodological aspects of application of electron diffraction of microcrystals are considered on the example of classical model protein: lysozyme.

Journal

Crystallography ReportsSpringer Journals

Published: Sep 1, 2021

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