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Electro-Optical Detection of Phage Antibody Interaction with Complementary Antigens of Herbaspirillum seropedicae Z78 Cells

Electro-Optical Detection of Phage Antibody Interaction with Complementary Antigens of... Complementary interactions in the antigen–antibody system have been assessed for the first time via electrooptical analysis with phage antibodies to the main antigens of the Herbaspirillum seropedicae Z78. It is shown that an electrooptical analyzer makes it possible to distinguish the presence or absence of specific interactions of phage antibodies with the main epitopes of a bacterial surface. The revealed relationships in the electrophysical parameters are in good agreement with the component composition of Herbaspirillum antigens and their topographic distribution, and they were also confirmed with electron microscopy and dot-analysis. It is shown that electrooptical analysis can be used to detect Herbaspirillum spp. with exposed antigens on its surface. The results can serve as a basis for the development of an express test for the rapid identification of these microorganisms. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Electro-Optical Detection of Phage Antibody Interaction with Complementary Antigens of Herbaspirillum seropedicae Z78 Cells

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Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2020
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S000368382001007X
Publisher site
See Article on Publisher Site

Abstract

Complementary interactions in the antigen–antibody system have been assessed for the first time via electrooptical analysis with phage antibodies to the main antigens of the Herbaspirillum seropedicae Z78. It is shown that an electrooptical analyzer makes it possible to distinguish the presence or absence of specific interactions of phage antibodies with the main epitopes of a bacterial surface. The revealed relationships in the electrophysical parameters are in good agreement with the component composition of Herbaspirillum antigens and their topographic distribution, and they were also confirmed with electron microscopy and dot-analysis. It is shown that electrooptical analysis can be used to detect Herbaspirillum spp. with exposed antigens on its surface. The results can serve as a basis for the development of an express test for the rapid identification of these microorganisms.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Jan 13, 2020

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