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Effect of the composition of the medium on the characteristics of polydisulfide bioantioxidants

Effect of the composition of the medium on the characteristics of polydisulfide bioantioxidants Characteristics of polydisulfides of gallic acid (PDSG), 2-amino-4-nitrophenol (PDSANP), and biuret (PDSB) depending on the composition of the aqueous medium were studied. In contrast to PDSANP and PDSB, there was oxidation of PDSG with accumulation of products of polydisulfide transformation in the medium. The rate of PDSG autoxidation depended on pH on the concentration of polydisulfide and buffers and increased at high pH. The rate of oxidation significantly increased in the presence of DMPA, ethanol, or CTAB (surfactant). Decreasing the pH of the solution and adding ovalbumin and/or Triton X-100 to the medium can decrease the rate of autoxidation of PDSG in an aqueous medium. Exogenous H2O2 inhibited the oxidation of PDSG. The secondary structure of catalase was changed by PDSG. Electrical conductance of PDSG and PDSANP solutions was studied. Possible mechanisms of PDSG autoxidation and polydisulfides-protein interaction due to forces of cooperative electrostatic interaction, thiol-disulfide exchanges, and nucleophilic replacements were discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Effect of the composition of the medium on the characteristics of polydisulfide bioantioxidants

Applied Biochemistry and Microbiology , Volume 36 (4) – Oct 27, 2007

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References (20)

Publisher
Springer Journals
Copyright
Copyright © 2000 by Eremin
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1007/BF02738048
Publisher site
See Article on Publisher Site

Abstract

Characteristics of polydisulfides of gallic acid (PDSG), 2-amino-4-nitrophenol (PDSANP), and biuret (PDSB) depending on the composition of the aqueous medium were studied. In contrast to PDSANP and PDSB, there was oxidation of PDSG with accumulation of products of polydisulfide transformation in the medium. The rate of PDSG autoxidation depended on pH on the concentration of polydisulfide and buffers and increased at high pH. The rate of oxidation significantly increased in the presence of DMPA, ethanol, or CTAB (surfactant). Decreasing the pH of the solution and adding ovalbumin and/or Triton X-100 to the medium can decrease the rate of autoxidation of PDSG in an aqueous medium. Exogenous H2O2 inhibited the oxidation of PDSG. The secondary structure of catalase was changed by PDSG. Electrical conductance of PDSG and PDSANP solutions was studied. Possible mechanisms of PDSG autoxidation and polydisulfides-protein interaction due to forces of cooperative electrostatic interaction, thiol-disulfide exchanges, and nucleophilic replacements were discussed.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Oct 27, 2007

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