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Effect of saccharides on plasma mediated haemagglutination and in vitro phagocytosis by haemocytes of the snailLymnaea stagnalis

Effect of saccharides on plasma mediated haemagglutination and in vitro phagocytosis by... To study the role of lectins in the defence system of the snailLymnaea stagnalis, saccharides were tested for their capacity to inhibit plasma-mediated haemagglutination of trypsinised and fixed mouse red blood cells (M-rbc) and fixed rabbit red blood cells (R-rbc).d-Arabinose,N-acetylneuraminic acid, fucoidan andd-melezitose were good agglutination inhibitors using both types of rbc.d-Fructose, however, stimulated haemagglutination. Based on these results, in vitro phagocytosis assays were performed withd-arabinose (an agglutination inhibitor) andd-fructose (an agglutination stimulator). In co-incubation experiments both sugars inhibited adherence and phagocytosis of M-rbc in a concentration-dependent manner. Adherence and phagocytosis of R-rbc was stimulated at the lowest concentrations of D-arabinose and most concentrations ofd-fructose. Preincubation experiments showed decreased adherence and phagocytosis after preincubation of M-rbc with either sugar. Adherence and phagocytosis of R-rbc was reduced after preincubation of haemocytes withd-fructose. Preincubation of R-rbc had little effect. Our results support former studies indicating the presence of lectins both in the snail plasma and on the haemocytes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Effect of saccharides on plasma mediated haemagglutination and in vitro phagocytosis by haemocytes of the snailLymnaea stagnalis

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Publisher
Springer Journals
Copyright
Copyright © 1998 by Springer-Verlag London Limited
Subject
Medicine & Public Health; Hematology; Oncology; Pathology
eISSN
1433-2973
DOI
10.1007/BF02628104
Publisher site
See Article on Publisher Site

Abstract

To study the role of lectins in the defence system of the snailLymnaea stagnalis, saccharides were tested for their capacity to inhibit plasma-mediated haemagglutination of trypsinised and fixed mouse red blood cells (M-rbc) and fixed rabbit red blood cells (R-rbc).d-Arabinose,N-acetylneuraminic acid, fucoidan andd-melezitose were good agglutination inhibitors using both types of rbc.d-Fructose, however, stimulated haemagglutination. Based on these results, in vitro phagocytosis assays were performed withd-arabinose (an agglutination inhibitor) andd-fructose (an agglutination stimulator). In co-incubation experiments both sugars inhibited adherence and phagocytosis of M-rbc in a concentration-dependent manner. Adherence and phagocytosis of R-rbc was stimulated at the lowest concentrations of D-arabinose and most concentrations ofd-fructose. Preincubation experiments showed decreased adherence and phagocytosis after preincubation of M-rbc with either sugar. Adherence and phagocytosis of R-rbc was reduced after preincubation of haemocytes withd-fructose. Preincubation of R-rbc had little effect. Our results support former studies indicating the presence of lectins both in the snail plasma and on the haemocytes.

Journal

Comparative Clinical Pathology – Springer Journals

Published: May 3, 2007

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