Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Effect of human amniotic membrane on anastomosis leakage in dog model

Effect of human amniotic membrane on anastomosis leakage in dog model One of the major concerns in rectal cancer surgery and complicated anastomosis is anastomosis breakdown and leakage. For decades, radiation (as neoadjuvant or adjuv ant therapy) was known as a risk factor for anastomosis leakage. Diverting loop ileostomy has been usually employed to divert the fecal flow and protect complicated anastomosis. However, there is a high rate of complications related to diverting loop ileostomy and its closure. This experimental study was carried out to investigate the effect of human amniotic membrane on colonic anastomosis in dogs that underwent preoperative radiation therapy. In this prospective controlled animal trial study, a total of ten male cross-breed dogs aged 6–8 months and weighing 10–15 kg were randomly assigned in to two groups of five dogs. Group 1 with five dogs received 10 Gy of external radiation preoperatively, while the second didn’t receive radiation. After anesthesia and exploration, about 8 cm above the dentate line, the bowel was cut and end-to-end anastomosis was done in two layers hand sewn technique. HAM measuring 2.5 cm × 2.5 cm was wrapped around the anastomosis and sutured with vicryl 3-0. In each group, one of the dogs died. The non-parametric Mann—Whitney test was used to compare the two groups. Two-tailed P value <0.05 was considered statistically significant. Cross sections of the healed anastomosis were scored according to modified scoring system. Histological evaluation of both groups demonstrated ulceration of the mucosa with infiltration of the acute and chronic inflammatory cells as well as granulation tissue formation. The healing starts by fibroblast proliferation and collagen deposition. A thin fibrotic tissue was formed between the serosa and patch graft. (P > 0.05, i.e., healing in both irradiated and non-irradiated groups was the same). Unfavorable effects of radiotherapy and loop ileostomy may be resolved with application of human amniotic membrane, which influences anastomosis healing and eliminates the need for ileostomy insertion. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Effect of human amniotic membrane on anastomosis leakage in dog model

Download PDF
6 pages

Loading next page...
 
/lp/springer-journals/effect-of-human-amniotic-membrane-on-anastomosis-leakage-in-dog-model-V0JSFb70ub
Publisher
Springer Journals
Copyright
Copyright © 2016 by Springer-Verlag London
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1618-565X
DOI
10.1007/s00580-016-2313-2
Publisher site
See Article on Publisher Site

Abstract

One of the major concerns in rectal cancer surgery and complicated anastomosis is anastomosis breakdown and leakage. For decades, radiation (as neoadjuvant or adjuv ant therapy) was known as a risk factor for anastomosis leakage. Diverting loop ileostomy has been usually employed to divert the fecal flow and protect complicated anastomosis. However, there is a high rate of complications related to diverting loop ileostomy and its closure. This experimental study was carried out to investigate the effect of human amniotic membrane on colonic anastomosis in dogs that underwent preoperative radiation therapy. In this prospective controlled animal trial study, a total of ten male cross-breed dogs aged 6–8 months and weighing 10–15 kg were randomly assigned in to two groups of five dogs. Group 1 with five dogs received 10 Gy of external radiation preoperatively, while the second didn’t receive radiation. After anesthesia and exploration, about 8 cm above the dentate line, the bowel was cut and end-to-end anastomosis was done in two layers hand sewn technique. HAM measuring 2.5 cm × 2.5 cm was wrapped around the anastomosis and sutured with vicryl 3-0. In each group, one of the dogs died. The non-parametric Mann—Whitney test was used to compare the two groups. Two-tailed P value <0.05 was considered statistically significant. Cross sections of the healed anastomosis were scored according to modified scoring system. Histological evaluation of both groups demonstrated ulceration of the mucosa with infiltration of the acute and chronic inflammatory cells as well as granulation tissue formation. The healing starts by fibroblast proliferation and collagen deposition. A thin fibrotic tissue was formed between the serosa and patch graft. (P > 0.05, i.e., healing in both irradiated and non-irradiated groups was the same). Unfavorable effects of radiotherapy and loop ileostomy may be resolved with application of human amniotic membrane, which influences anastomosis healing and eliminates the need for ileostomy insertion.

Journal

Comparative Clinical Pathology – Springer Journals

Published: Aug 26, 2016

References

  • Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model
    Abramov, Y; Golden, B; Sullivan, M; Botros, SM; Miller, JJ; Alshahrour, A
  • Long-term results of intersphincteric resection for low rectal cancer
    Chamlou, R; Parc, Y; Simon, T
  • Effects of intraperitoneal chemotherapy and GM-CSF on anastomotic healing: an experimental study in rats
    Erdem, E; Dinç, S; Erdem, D
  • Complications of loop lleostomy
    Felnbeg, SM; McLeod, RS; Cohen, Z
  • Wound healing after radiation therapy: review of the literature
    Haubner, F; Ohmann, E; Pohl, F; Strutz, J; Gassner, HG
  • The skin: its structure and response to ionizing radiation
    Hopewell, JW
  • Cryopreserved human amniotic membrane for soft tissue repair in rats
    Kesting, MR; Loeffelbein, DJ; Steinstraesser, L; Muecke, T; Demtroeder, C; Sommerer, F
  • The role of allogenic amniotic membrane in burn treatment
    Kesting, MR; Wolff, KD; Hohlweg-Majert, B; Steinstraesser, L
  • Preoperative radiation therapy produces an early and persistent reduction in colorectal anastomotic blood flow
    Milsom, J; Senagore, A; Walshaw, R; Mostosky, U; Wang, P; Johnson, W; Chaudry, I
  • Effect of fresh human amniotic membrane dressing on graft take in patients with chronic burn wounds compared with conventional methods
    Mohammadi, AA; Seyed Jafari, SM; Kiasat, M; Tavakkolian, AR; Imani, MT; Ayaz, M
  • Intraoral defect coverage with prelaminated epigastric fat flaps with human amniotic membrane in rats
    Mucke, T; Loeffelbein, DJ; Holzle, F; Slotta-Huspenina, J; Borgmann, A; Kanatas, AN
  • The effect of selenium and/or vitamin E treatments on radiation-induced intestinal injury in rats
    Mutlu-Turkoglu, U; Erbil, Y; Oztezcan, S; Olgac, V; Toker, G; Uysal, M
  • The effects of human amniotic membrane on healing of colonic anastomosis in dogs
    Najibpour, N; Jahantab, MG; Hosseinzadeh, M; Roshanravan, R; Moslemi, S; Rahimikazerooni, S
  • Effect of electromagnetic fields and early postoperative 5-fluorouracil on the healing of colonic anastomoses
    Nayci, A; Cakmak, M; Renda, N
  • Properties of the amniotic membrane for potential use in tissue engineering
    Niknejad, H; Peirovi, H; Jorjani, M; Ahmadiani, A; Ghanavi, J; Seifalian, AM
  • Fibrin glue does not improve healing of gastrointestinal anastomoses: a systematic review
    Nordentoft, T; Pommergaar, HS; Rosenberg, J; Achiam, MP
  • Comparison of abdominoperineal resection and low anterior resection in lower and middle rectal cancer
    Omidvari, S; Hamedi, SH; Mohammadianpanah, M; Razzaghi, S; Mosalaei, A; Ahmadloo, N
  • Radiation processed amniotic membranes in the treatment of non-healing ulcers of different etiologies
    Singh, R; Chouhan, US; Purohit, S; Gupta, P; Kumar, P; Kumar, A; Chacharkar, MP; Kachhawa, D; Ghiya, BC
  • Effects of amniotic membrane on the healing of normal and high-risk colonic anastomoses in rats
    Uludag, M; Citgez, B; Ozkaya, O
  • Covering the colon anastomoses with amniotic membrane prevents the negative effects of early intraperitoneal 5-FU administration on anastomotic healing
    Uludag, M; Ozdilli, K; Citgez, B; Yetkin, G; Ipcioglu, OM; Ozcan, O
  • The intestinal complications of radiotherapy
    Wellwood, J; Jackson, B