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Effect of cooking duration on the antioxidant and antihyperglycemic properties of Andrographis paniculata (king of bitters) leaves in high sucrose diet-fed fruit flies (Drosophila melanogaster)

Effect of cooking duration on the antioxidant and antihyperglycemic properties of Andrographis... King of bitters (Andrographis paniculata) leaf is often prepared by infusion of the leaves, or cooking at different time, for folkloric use in the management of several diseases including type 2 diabetes. This study evaluated the effects of cooking duration on the antioxidant and antihyperglycemic properties of Andrographis paniculata on high sucrose diet fed fruit flies (Drosophila melanogaster). A. paniculata leaves were pulverized and divided into three portion; one was subjected to water infusion and the other two portions were cooked for 5 and 10 min respectively. Wild-type D. melanogaster were raised for 14 days on high sucrose diet (HSD) supplemented with A. paniculata at different cooking intervals. Thereafter, the mortality rate, negative geotaxis, body weight, antioxidant indices, thiobarbituric reactive substances (TBARS), total thiol, superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), and carbohydrate metabolism indices (total glucose, α-amylase, α-glucosidase) were determined. The result also revealed that the flies fed with HSD diet showed significant reductions in negative geotaxis accompanied by elevation in mortality rate, TBARS level, glucose concentration, and α-amylase and α-glucosidase activities, as well as reductions in total thiol level, SOD, catalase, and GST activities. However, flies fed A. paniculata supplemented diet significantly reversed the observed impairments with 5-min cooking time exhibiting the best antihyperglycemic and antioxidant effects. Varied amounts of gallic acid, catechin, ferulic acid, rutin, quercetin, and kaempferol were identified and quantified via HPLC analysis in the uncooked and cooked samples. Thus, this study established that 5 min cooking of A. paniculata could preserve and enhance the phenolic phytochemicals, antihyperglycemic, and antioxidant properties of A. paniculata. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Effect of cooking duration on the antioxidant and antihyperglycemic properties of Andrographis paniculata (king of bitters) leaves in high sucrose diet-fed fruit flies (Drosophila melanogaster)

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Publisher
Springer Journals
Copyright
Copyright © The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
eISSN
1618-565X
DOI
10.1007/s00580-022-03393-7
Publisher site
See Article on Publisher Site

Abstract

King of bitters (Andrographis paniculata) leaf is often prepared by infusion of the leaves, or cooking at different time, for folkloric use in the management of several diseases including type 2 diabetes. This study evaluated the effects of cooking duration on the antioxidant and antihyperglycemic properties of Andrographis paniculata on high sucrose diet fed fruit flies (Drosophila melanogaster). A. paniculata leaves were pulverized and divided into three portion; one was subjected to water infusion and the other two portions were cooked for 5 and 10 min respectively. Wild-type D. melanogaster were raised for 14 days on high sucrose diet (HSD) supplemented with A. paniculata at different cooking intervals. Thereafter, the mortality rate, negative geotaxis, body weight, antioxidant indices, thiobarbituric reactive substances (TBARS), total thiol, superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), and carbohydrate metabolism indices (total glucose, α-amylase, α-glucosidase) were determined. The result also revealed that the flies fed with HSD diet showed significant reductions in negative geotaxis accompanied by elevation in mortality rate, TBARS level, glucose concentration, and α-amylase and α-glucosidase activities, as well as reductions in total thiol level, SOD, catalase, and GST activities. However, flies fed A. paniculata supplemented diet significantly reversed the observed impairments with 5-min cooking time exhibiting the best antihyperglycemic and antioxidant effects. Varied amounts of gallic acid, catechin, ferulic acid, rutin, quercetin, and kaempferol were identified and quantified via HPLC analysis in the uncooked and cooked samples. Thus, this study established that 5 min cooking of A. paniculata could preserve and enhance the phenolic phytochemicals, antihyperglycemic, and antioxidant properties of A. paniculata.

Journal

Comparative Clinical PathologySpringer Journals

Published: Dec 1, 2022

Keywords: King of bitters; α-Amylase; α-Glucosidase; High sucrose diet; Cooking duration; Fruit flies

References