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- Publisher
- Springer Journals
- Copyright
- Copyright
- Subject
- Philosophy; Philosophy of Biology; Evolutionary Biology
- ISSN
- 0001-5342
- eISSN
- 1572-8358
- DOI
- 10.1007/BF00713555
- Publisher site
- See Article on Publisher Site
Abstract
Increasing interest has been paid to applications of fluorescence measurements to analyze physiological mechanisms in living cells. However, few studies have taken advantage of DNA quantification by fluorometry for dynamic assessment of chromatin organization as well as cell motion during the cell cycle. This approach involves both optimal conditions for DNA staining and cell tracking methods. In this context, this report describes a stoichiometric method for nuclear DNA specific staining, using the bisbenzimidazole dye Hoechst 33342 associated with verapamil, a calcium membrane channel blocker. This method makes it possible to correlate variations of nuclear DNA content with cell motion in cells that are maintained alive. Motion measurement is the second goal of this paper and it explains the snake-spline method, and the associated cell following method.
Journal
Acta Biotheoretica – Springer Journals
Published: Nov 12, 2004