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DNA-functionalized dye-loaded carbon dots: ultrabright FRET platform for ratiometric detection of Hg(II) in serum samples and cell microenvironment

DNA-functionalized dye-loaded carbon dots: ultrabright FRET platform for ratiometric detection of... The dual-emission carbon dots (CDs) are used to fabricate an ultra-sensitive and highly selective fluorescence resonance energy transfer (FRET) aptameric-sensor for the quantitation of Hg(II). The designed strategy worked based on hybridization between T- rich ssDNA(S1) immobilized on CDs and AuNPs modified with complementary aptamer (AuNPs-S2) and Rhodamine B (RB) as an extra internal reference. Under optimized experimental conditions, the intensity ratio of I /I shows a good linear rela- 580 668 −18 −5 −19 tionship with the Hg(II) concentration in concentration range of 1.0 × 10 to 50.0 × 10 M with detection limit of 5 × 10 M. The proposed FRET aptasensor showed high selectivity for Hg(II) determination even in the presence of other metal ions with higher concentration as high as 1000 times. Furthermore, the RB-CDs probe was demonstrated to be efficient for MDA-MB 231 cell imaging. The bifunctional signaling probe exhibits impressive simplicity, convenience, and low detection time and the proposed sensor can be applied in biomedicine study, food safety, and environmental protection. . . . . . Keywords Ratiometric Dual emission carbon dots Hg(II) Aptasensor Cellular imaging Fluorescence resonance energy transfer (FRET) Rhodamine B Introduction and immunity. Accordingly, it is crucial to accentuate the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Ionics Springer Journals

DNA-functionalized dye-loaded carbon dots: ultrabright FRET platform for ratiometric detection of Hg(II) in serum samples and cell microenvironment

Ionics , Volume OnlineFirst – Apr 19, 2019

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References (54)

Publisher
Springer Journals
Copyright
Copyright © 2019 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Chemistry; Electrochemistry; Renewable and Green Energy; Optical and Electronic Materials; Condensed Matter Physics; Energy Storage
ISSN
0947-7047
eISSN
1862-0760
DOI
10.1007/s11581-019-02999-2
Publisher site
See Article on Publisher Site

Abstract

The dual-emission carbon dots (CDs) are used to fabricate an ultra-sensitive and highly selective fluorescence resonance energy transfer (FRET) aptameric-sensor for the quantitation of Hg(II). The designed strategy worked based on hybridization between T- rich ssDNA(S1) immobilized on CDs and AuNPs modified with complementary aptamer (AuNPs-S2) and Rhodamine B (RB) as an extra internal reference. Under optimized experimental conditions, the intensity ratio of I /I shows a good linear rela- 580 668 −18 −5 −19 tionship with the Hg(II) concentration in concentration range of 1.0 × 10 to 50.0 × 10 M with detection limit of 5 × 10 M. The proposed FRET aptasensor showed high selectivity for Hg(II) determination even in the presence of other metal ions with higher concentration as high as 1000 times. Furthermore, the RB-CDs probe was demonstrated to be efficient for MDA-MB 231 cell imaging. The bifunctional signaling probe exhibits impressive simplicity, convenience, and low detection time and the proposed sensor can be applied in biomedicine study, food safety, and environmental protection. . . . . . Keywords Ratiometric Dual emission carbon dots Hg(II) Aptasensor Cellular imaging Fluorescence resonance energy transfer (FRET) Rhodamine B Introduction and immunity. Accordingly, it is crucial to accentuate the

Journal

IonicsSpringer Journals

Published: Apr 19, 2019

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