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Different gene expression of the constitutive nitric oxide synthase (NOS-3) in bull epididymis and vas deferens

Different gene expression of the constitutive nitric oxide synthase (NOS-3) in bull epididymis... To determine the presence of constitutive nitric oxide synthase (NOS-3) mRNA in the caput, corpus, cauda epididymis, and vas deferens of adult bull, semiquantitative reverse transcription-polymerase chain reaction was performed on total RNAs isolated from those tissues. Expression of NOS-3 gene was exhibited in the samples of vas deferens and three parts of epididymis (caput, corpus, and cauda). Of course, the NOS-3 gene was detected in the part of the corpus weakly and was significant in comparison to other parts. NOS-3 mRNA in the part of cauda was nonsignificantly more than in other parts of the epididymis. Expression of this gene was significantly elevated in the two parts of the epididymis (caput and cauda) when compared with the vas deferens samples. These data could be the evidence for a different contribution of NOS-produced NO in the functions of epididymal segments and vas deferens. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Different gene expression of the constitutive nitric oxide synthase (NOS-3) in bull epididymis and vas deferens

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References (27)

Publisher
Springer Journals
Copyright
Copyright © 2008 by Springer-Verlag London Limited
Subject
Medicine & Public Health; Oncology ; Hematology; Pathology
eISSN
1618-565X
DOI
10.1007/s00580-008-0756-9
Publisher site
See Article on Publisher Site

Abstract

To determine the presence of constitutive nitric oxide synthase (NOS-3) mRNA in the caput, corpus, cauda epididymis, and vas deferens of adult bull, semiquantitative reverse transcription-polymerase chain reaction was performed on total RNAs isolated from those tissues. Expression of NOS-3 gene was exhibited in the samples of vas deferens and three parts of epididymis (caput, corpus, and cauda). Of course, the NOS-3 gene was detected in the part of the corpus weakly and was significant in comparison to other parts. NOS-3 mRNA in the part of cauda was nonsignificantly more than in other parts of the epididymis. Expression of this gene was significantly elevated in the two parts of the epididymis (caput and cauda) when compared with the vas deferens samples. These data could be the evidence for a different contribution of NOS-produced NO in the functions of epididymal segments and vas deferens.

Journal

Comparative Clinical PathologySpringer Journals

Published: Sep 9, 2008

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