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DhLHY, a Circadian Expressed Gene of Doritaenopsis Hybrid, Promotes Floral Transition in Low Temperature, but Postpones Flowering in Overexpressed Transgenic Arabidopsis

DhLHY, a Circadian Expressed Gene of Doritaenopsis Hybrid, Promotes Floral Transition in Low... LATE ELONGATED HYPOCOTYL (LHY) gene is well known to be the core circadian function in Arabidopsis. In this study, DhLHY was obtained from Doritaenopsis ‘Tinny Tender’ (Doritaenopsis Happy smile × Happy valentine), and the sequence contained 2594 base pair (bp) in whole with an open reading frame (ORF) of 1962 bp encoding 653 amino acids. The putative protein of DhLHY included a HTH myb domain and a DNA binding site shared with the reported LHY family. Aligment of amino acid (aa) showed that DhLHY shared high similarity of LHY homologs from other species. Real-time quantitative reversed transcript polymerase chain reaction (qRT-PCR) analysis indicated that DhLHY was a morning-expressed circadian gene, and expressed greater in floral induction under low temperature. DhLHY was expressed both in vegetative and reproductive organs, and the highest level of its transcripts were in the pedicle and stem. Further functional study of DhLHY-overexpressed in Arobidopsis thaliana indicated the delayed bolting time and increased leaf length in transgenic plants. Results suggested that DhLHY participated in the circadian regulation and was response to the low temperature of floral induction in Doritaenopsis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Tropical Plant Biology Springer Journals

DhLHY, a Circadian Expressed Gene of Doritaenopsis Hybrid, Promotes Floral Transition in Low Temperature, but Postpones Flowering in Overexpressed Transgenic Arabidopsis

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Publisher
Springer Journals
Copyright
Copyright © Springer Science+Business Media, LLC, part of Springer Nature 2019
Subject
Life Sciences; Plant Sciences; Plant Genetics and Genomics; Plant Breeding/Biotechnology; Plant Ecology; Transgenics
ISSN
1935-9756
eISSN
1935-9764
DOI
10.1007/s12042-019-09250-2
Publisher site
See Article on Publisher Site

Abstract

LATE ELONGATED HYPOCOTYL (LHY) gene is well known to be the core circadian function in Arabidopsis. In this study, DhLHY was obtained from Doritaenopsis ‘Tinny Tender’ (Doritaenopsis Happy smile × Happy valentine), and the sequence contained 2594 base pair (bp) in whole with an open reading frame (ORF) of 1962 bp encoding 653 amino acids. The putative protein of DhLHY included a HTH myb domain and a DNA binding site shared with the reported LHY family. Aligment of amino acid (aa) showed that DhLHY shared high similarity of LHY homologs from other species. Real-time quantitative reversed transcript polymerase chain reaction (qRT-PCR) analysis indicated that DhLHY was a morning-expressed circadian gene, and expressed greater in floral induction under low temperature. DhLHY was expressed both in vegetative and reproductive organs, and the highest level of its transcripts were in the pedicle and stem. Further functional study of DhLHY-overexpressed in Arobidopsis thaliana indicated the delayed bolting time and increased leaf length in transgenic plants. Results suggested that DhLHY participated in the circadian regulation and was response to the low temperature of floral induction in Doritaenopsis.

Journal

Tropical Plant BiologySpringer Journals

Published: Jun 16, 2020

References