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Development of the St/J and V Genome Specific Molecular Marker Based on 5s rDNA Polymorphism in Thinopyrum bessarabicum, Pseudoroegneria spicata, and Dasypyrum villosum

Development of the St/J and V Genome Specific Molecular Marker Based on 5s rDNA Polymorphism in... Nontranscribed spacers (NTS) of 5S rDNA are often polymorphic in closely related species and even in the same genome. The polymorphism of 5S rDNA NTS was shown between genomes St, J, and V of Triticeae species Thinopyrum bessarabicum, Pseudoroegneria spicata, and Dasypyrum villosum, respectively. A molecular genetic marker was designed based on the 5S rDNA NTS polymorphism that allows identification of the St, J, and V genomes. We designed a pair of primers that correspond to the conserved regions of 5S rDNA NTS between the genomes studied. The PCR amplicon length is 158 bp, 171 bp, and 172 bp for V, St, and J genomes, respectively. The fragment of the St genome is characterized by the SmiM I restriction site that enables its differentiation from the J genome fragment that lacks this site. The developed marker showed its efficiency for verification of germplasm accessions and the study of allopolyploids. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Moscow University Biological Sciences Bulletin Springer Journals

Development of the St/J and V Genome Specific Molecular Marker Based on 5s rDNA Polymorphism in Thinopyrum bessarabicum, Pseudoroegneria spicata, and Dasypyrum villosum

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References (24)

Publisher
Springer Journals
Copyright
Copyright © 2018 by Allerton Press, Inc.
Subject
Life Sciences; Biochemistry, general; Cell Biology; Life Sciences, general; Plant Sciences; Zoology
ISSN
0096-3925
eISSN
1934-791X
DOI
10.3103/S0096392518010017
Publisher site
See Article on Publisher Site

Abstract

Nontranscribed spacers (NTS) of 5S rDNA are often polymorphic in closely related species and even in the same genome. The polymorphism of 5S rDNA NTS was shown between genomes St, J, and V of Triticeae species Thinopyrum bessarabicum, Pseudoroegneria spicata, and Dasypyrum villosum, respectively. A molecular genetic marker was designed based on the 5S rDNA NTS polymorphism that allows identification of the St, J, and V genomes. We designed a pair of primers that correspond to the conserved regions of 5S rDNA NTS between the genomes studied. The PCR amplicon length is 158 bp, 171 bp, and 172 bp for V, St, and J genomes, respectively. The fragment of the St genome is characterized by the SmiM I restriction site that enables its differentiation from the J genome fragment that lacks this site. The developed marker showed its efficiency for verification of germplasm accessions and the study of allopolyploids.

Journal

Moscow University Biological Sciences BulletinSpringer Journals

Published: Dec 14, 2017

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