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Development of Osteoplastic Material Impregnated with Plasmid Encoding Bone Morphogenetic Protein-2

Development of Osteoplastic Material Impregnated with Plasmid Encoding Bone Morphogenetic Protein-2 The biological properties of a new gene-activated osteoplastic material based on chitosan sponges impregnated with plasmids with the gene BMP-2 for bone morphogenetic protein-2 have been studied. The optimal conditions for the transfection of multipotent mesenchymal stromal cells with TurboFect reagent are selected. It is shown that the in vitro osteoinductive effect of the developed material is associated with an increase in the expression of osteogenic markers in mesenchymal stromal cells. The designed gene-activated material can be used to repair bone defects. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Development of Osteoplastic Material Impregnated with Plasmid Encoding Bone Morphogenetic Protein-2

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Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2021. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 7, pp. 818–822. © Pleiades Publishing, Inc., 2021. Russian Text © The Author(s), 2020, published in Biotekhnologiya, 2020, Vol. 36, No. 4, pp. 59–64.
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/s000368382107005x
Publisher site
See Article on Publisher Site

Abstract

The biological properties of a new gene-activated osteoplastic material based on chitosan sponges impregnated with plasmids with the gene BMP-2 for bone morphogenetic protein-2 have been studied. The optimal conditions for the transfection of multipotent mesenchymal stromal cells with TurboFect reagent are selected. It is shown that the in vitro osteoinductive effect of the developed material is associated with an increase in the expression of osteogenic markers in mesenchymal stromal cells. The designed gene-activated material can be used to repair bone defects.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Dec 1, 2021

Keywords: gene-activated material; plasmids; BMP-2; gene therapy; bone regeneration

References