Access the full text.
Sign up today, get DeepDyve free for 14 days.
C Silvar, J Duncan, D Cooke, N Williams, J Díaz, F Merino (2005)
Development of specific PCR primers for identification and detection of Phytophthora capsici LeonEur J Plant Pathol, 112
H Haubruck, C Disela, P Wagner, D Gallwitz (1987)
The ras-related Ypt protein is an ubiquitous eukaryotic protein: isolation and sequence analysis of mouse cDNA clones highly homologous to the yeast Ypt1 geneEMBO J, 6
C Pavón, M Babadoost, K Lambert (2008)
Quantification of Phytophthora capsici oospores in soil by sieving-centrifugation and real-time polymerase chain reactionPlant Dis, 92
K Jackson, J Yin, P Ji (2012)
Sensitivity of Phytophthora capsici on vegetable crops in Georgia to mandipropamid, dimethomorph and cyazofamidPlant Dis, 96
L Schena, J Duncan, D Cooke (2008)
Development and application of a PCR-based ‘molecular tool box’ for the identification of Phytophthora species damaging forests and natural ecosystemsPlant Pathol, 57
C Silvar, J Díaz, F Merino (2005)
Real-time polymerase chain reaction quantification of Phytophthora capsici in different pepper genotypesPhytopathology, 95
MK Hausbeck, KH Lamour (2004)
Phytophthora capsici on vegetable crops: research progress and management challengesPlant Dis, 88
L Schena, KJD Hughes, DEL Cooke (2006)
Detection and quantification of Phytophthora ramorum, P. kernoviae, P. citricola and P. quercina in symptomatic leaves by multiplex real–time PCRMol Plant Pathol, 7
Z Zhang, Y Li, H Fan, Y Wang, X Zheng (2006)
Molecular detection of Phytophthora capsici in infected plant tissues, soil and waterPlant Pathol, 55
M Li, T Asano, H Suga, K Kageyama (2011)
Development of multiplex PCR for the detection of Phytophthora nicotianae and P. cactorum, and a survey of their occurrence in the main strawberry production areas of JapanPlant Dis, 95
C Trout, J Ristaino, M Madritch, T Wangsomboondee (1997)
Rapid detection of Phytophthora infestans in late blight-infected potato and tomato using PCRPlant Dis, 81
FN Martin, ZG Abad, Y Balci, K Ivors (2012)
Identification and detection of phytophthora: reviewing our progress, identifying our needsPlant Dis, 96
P Tooley, B Bunyard, M Carras, E Hatziloukas (1997)
Development of PCR primers from internal transcribed spacer region 2 for detection of Phytophthora species infecting potatoesAppl Environ Microbiol, 63
JB Ristaino, SA Johnston (1999)
Ecologically based approaches to management of Phytophthora blight on bell pepperPlant Dis, 83
Z Wang, DB Langston, AS Csinos, RD Gitaitis, RR Walcott, P Ji (2009)
Development of an improved isolation approach and simple sequence repeat markers to characterize Phytophthora capsici populations in irrigation ponds in southern GeorgiaAppl Environ Microbiol, 75
J Meng, Y Wang (2010)
Rapid detection of Phytophthora nicotianae in infected tobacco tissues and soil samples based on its Ypt1 geneJ Phytopathol, 158
LD Padley, EA Kabelka, PD Roberts, R French (2008)
Evaluation of Cucurbita pepo accessions for crown rot resistance to isolates of Phytophthora capsiciHortSci, 43
Phytophthora blight caused by Phytophthora capsici is a serious disease affecting pepper production and other important vegetable crop. This study developed a species-specific PCR assay for the rapid and accurate detection of P. capsici in infected plant tissues, soil, and water. The PCR primers were designed based on Ras-related protein (Ypt1) gene, and 236 isolates representing 12 species of Phytophthora and 26 fungal species of plant pathogens were used to test the specificity of the primers. PCR amplification with the species-specific primer pair resulted in products of 364 bp solely from all isolates of P. capsici that was not amplified from any other isolates tested. The detection sensitivity of the species-specific primer pair Pc1F/Pc1R was 10 pg of genomic DNA. This sensitivity increased 1000-fold to 10 fg by developing a nested PCR procedure that uses Pc1F/Pc1R as first-round primers combined with Pc1F/Pc2R as a second round primers. The PCR assay can also be used to detect P. capsici in naturally infected plant tissues, soil, and water. The PCR-based methods developed here could simplify both plant disease diagnosis and pathogen monitoring and aid in plant disease management.
Australasian Plant Pathology – Springer Journals
Published: Dec 14, 2012
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.