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References (20)
-
呂昀陞, 鄧文玲, 楊雯馨, 吳雅芳, 鄭安秀, 徐世典, 曾國欽 (2010)
Multiplex Polymerase Chain Reaction for Simultaneous Detection of Xanthomonas campestris pv. campestris and X. campestris pv. raphani, 19
-
J. Vicente, E. Holub (2013)
Xanthomonas campestris pv. campestris (cause of black rot of crucifers) in the genomic era is still a worldwide threat to brassica crops.Molecular plant pathology, 14 1
-
田村 勝徳, 瀧川 雄一, 露無 慎二, 後藤 正夫 (1994)
Bacterial Spot of Crucifers Caused by Xanthomonas campestris pv. raphani., 60
-
L. Trindade, E. Marques, D. Lopes, M. Ferreira (2007)
Development of a molecular method for detection and identification of Xanthomonas campestris pv. viticolaSumma Phytopathologica, 33
-
M. Moffett, D. Trimboli, I. Bonner (1976)
A bacterial leaf spot disease of several brassica varietiesAustralian Plant Pathology Society Newsletter, 5
-
Y. Park, Byoung-Moo Lee, Jang Ho-Hahn, Gil-Bok Lee, Dong-Suk Park (2004)
Sensitive and specific detection of Xanthomonas campestris pv campestris by PCR using species-specific primers based on hrpF gene sequences.Microbiological research, 159 4
-
J. Cruz, R. Tenreiro, L. Cruz (2015)
First Report of Xanthomonas campestris pv. raphani Causing Leaf Spot Disease of Brassica oleracea in Portugal.Plant disease, 99 2
-
A. Darling, B. Mau, N. Perna (2010)
progressiveMauve: Multiple Genome Alignment with Gene Gain, Loss and RearrangementPLoS ONE, 5
-
A. Alvarez, K. Lou (1985)
Rapid identification of Xanthomonas campestris pv. campestris by ELISA.Plant Disease, 69
-
J. Vicente, B. Everett, S. Roberts (2006)
Identification of Isolates that Cause a Leaf Spot Disease of Brassicas as Xanthomonas campestris pv. raphani and Pathogenic and Genetic Comparison with Related Pathovars.Phytopathology, 96 7
-
ML Moffett, D Trimboli, I Bonner (1976)
A bacterial leaf spot disease of several Brassica varietiesAustralas Plant Pathol, 5
-
H. White
Bacterial spot of Radish and Turnip.Phytopathology, 20
-
Y. Wu, S. Chen, S. Huang, A. Cheng (2007)
First report of bacterial leaf spot disease of crucifers caused by Xanthomonas campestris pv. raphani in Taiwan.Plant Pathology Bulletin, 16
-
N. Schaad, E. Postnikova, G. Lacy, A. Sechler, I. Agarkova, P. Stromberg, V. Stromberg, A. Vidaver (2006)
Emended classification of xanthomonad pathogens on citrus.Systematic and applied microbiology, 29 8
-
You-Xing Zhao, J. Damicone, D. Demezas, C. Bender (2000)
Bacterial Leaf Spot Diseases of Leafy Crucifers in Oklahoma Caused by Pathovars of Xanthomonas campestris.Plant disease, 84 9
-
A. Alvarez (2003)
Integrated approaches for detection of plant pathogenic bacteria and diagnosis of bacterial diseases.Annual review of phytopathology, 42
-
L. Chitarra, C. Langerak, J. Bergervoet, R. Bulk (2002)
Detection of the plant pathogenic bacterium Xanthomonas campestris pv. Campestris in seed extracts of Brassica sp. Applying fluorescent antibodies and flow cytometry.Cytometry, 47 2
-
E. Fargier, C. Manceau (2007)
Pathogenicity assays restrict the species Xanthomonas campestris into three pathovars and reveal nine races within X. campestris pv. campestrisPlant Pathology, 56
-
H. Koenraadt, J. Bilsen, S. Roberts (2005)
Comparative test of four semi-selective agar media for the detection of Xanthomonas campestris pv. campestris in brassica seeds.Seed Science and Technology, 33
-
T. Berg, L. Tesoriero, D. Hailstones (2005)
PCR‐based detection of Xanthomonas campestris pathovars in Brassica seedPlant Pathology, 54
- Publisher
- Springer Journals
- Copyright
- Copyright © 2019 by Australasian Plant Pathology Society Inc.
- Subject
- Life Sciences; Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology
- ISSN
- 0815-3191
- eISSN
- 1448-6032
- DOI
- 10.1007/s13313-019-0614-z
- Publisher site
- See Article on Publisher Site
Abstract
Leaf spot of Brassica is an important bacterial seed borne disease caused by X. campestris pv. raphani (Xcr), one of the three pathovars of Xanthomonas campestris species which makes significant economic loss throughout the world. A rapid and reliable detection method will be helpful for early diagnosis of the disease. To do that, we designed Xcr specific sequence-characterized amplified region (SCAR) markers from the Xcr specific genomic fragments, identified by aligning the whole genome sequences of several closely related bacterial pathovars and other bacterial species. Our primer set Xcr_59 produced Xcr specific amplicon of 679 bp in PCR assays. This primer pair was also capable of detecting Xcr strains directly from the artificially infected leaf samples prepared by suspending the diseased leaf area in sterile water without extracting bacterial DNA. This rapid, sensitive and reliable detection technique will be useful for adopting any firsthand plant protection measures against the disease.
Journal
Australasian Plant Pathology – Springer Journals
Published: Jan 25, 2019