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Development of a holder to avoid handling human crystalline lenses in biological assays

Development of a holder to avoid handling human crystalline lenses in biological assays PurposeThe importance of this study is to develop handling strategies for human lens studies, aiming at eye health. The aim of this study was to develop a lens holder to facilitate handling lenses having minimal contact with different surfaces while developing experimental protocols.MethodsWe devised a dissection technique to harvest the lens together with the iris and ciliary body, avoiding any contact with the lens. We also made a specific holder for the lens. Five human lenses were used and preserved in TC199 solution at 4 °C. To test the practicality of the new tool, we weighed the lenses and performed transmission spectroscopy to evaluate the holder’s interference during measurements.ResultsThe lens holder consists of a pair of anodized plaques with eight pins attached to one of the plaques. The lens is accommodated in the holder by attaching the ciliary muscle to the pins. The holder allows the lens to float inside it, preventing it from deforming, and keeping it intact during the experiment. Overall, the lens holder showed suitable handling in repeated measurement experiments of spectral transmission and daily measurement of the lens mass. In both analyses, the lenses were kept inside the holder without needing to remove them.ConclusionThe novelty of this new lens holder is that it prevents the lens from touching hard surfaces, which can damage the lens. Moreover, the differential of this holder is the possibility of carrying out analyzes on different pieces of equipment without needing to handle the lens. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Biomedical Engineering Springer Journals

Development of a holder to avoid handling human crystalline lenses in biological assays

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Publisher
Springer Journals
Copyright
Copyright © Sociedade Brasileira de Engenharia Biomedica 2021
ISSN
2446-4732
eISSN
2446-4740
DOI
10.1007/s42600-020-00115-2
Publisher site
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Abstract

PurposeThe importance of this study is to develop handling strategies for human lens studies, aiming at eye health. The aim of this study was to develop a lens holder to facilitate handling lenses having minimal contact with different surfaces while developing experimental protocols.MethodsWe devised a dissection technique to harvest the lens together with the iris and ciliary body, avoiding any contact with the lens. We also made a specific holder for the lens. Five human lenses were used and preserved in TC199 solution at 4 °C. To test the practicality of the new tool, we weighed the lenses and performed transmission spectroscopy to evaluate the holder’s interference during measurements.ResultsThe lens holder consists of a pair of anodized plaques with eight pins attached to one of the plaques. The lens is accommodated in the holder by attaching the ciliary muscle to the pins. The holder allows the lens to float inside it, preventing it from deforming, and keeping it intact during the experiment. Overall, the lens holder showed suitable handling in repeated measurement experiments of spectral transmission and daily measurement of the lens mass. In both analyses, the lenses were kept inside the holder without needing to remove them.ConclusionThe novelty of this new lens holder is that it prevents the lens from touching hard surfaces, which can damage the lens. Moreover, the differential of this holder is the possibility of carrying out analyzes on different pieces of equipment without needing to handle the lens.

Journal

Research on Biomedical Engineering – Springer Journals

Published: Jan 6, 2021

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