Access the full text.
Sign up today, get DeepDyve free for 14 days.
K. Dashtian, S. Hajati, M. Ghaedi (2020)
L-phenylalanine-imprinted polydopamine-coated CdS/CdSe n-n type II heterojunction as an ultrasensitive photoelectrochemical biosensor for the PKU monitoring.Biosensors & bioelectronics, 165
C. Scriver (2007)
The PAH gene, phenylketonuria, and a paradigm shiftHuman Mutation, 28
KM Sumaily (2017)
63.4International Journal of Health Sciences, 11
A. Dinu, C. Apetrei (2020)
A Review on Electrochemical Sensors and Biosensors Used in Phenylalanine ElectroanalysisSensors (Basel, Switzerland), 20
Xi-ming Mo, Ying Li, A. Tang, Ya-Ping Ren (2013)
Simultaneous determination of phenylalanine and tyrosine in peripheral capillary blood by HPLC with ultraviolet detection.Clinical biochemistry, 46 12
K. Sumaily, Ahmed Mujamammi (2017)
Phenylketonuria: A new look at an old topic, advances in laboratory diagnosis, and therapeutic strategiesInternational Journal of Health Sciences, 11
Naz Hafid, J. Christodoulou (2015)
Phenylketonuria: a review of current and future treatments.Translational pediatrics, 4 4
E. Vardy, A. Macdonald, Suzanne Ford, D. Hofman (2020)
Phenylketonuria, co‐morbidity, and ageing: A reviewJournal of Inherited Metabolic Disease, 43
K. Ikeda, E. Schiltz, T. Fujii, M. Takahashi, K. Mitsui, Y. Kodera, A. Matsushima, Y. Inada, G. Schulz, H. Nishimura (2005)
Phenylalanine ammonia-lyase modified with polyethylene glycol: Potential therapeutic agent for phenylketonuriaAmino Acids, 29
K. Wieder, N. Palczuk, T. Es, Frank Davis (1979)
Some properties of polyethylene glycol:phenylalanine ammonia-lyase adducts.The Journal of biological chemistry, 254 24
P. Jafari, S. Beigi, F. Yousefi, Sajjad Aghabalazadeh, Maryam Mousavizadegan, M. Hosseini, S. Hosseinkhani, M. Ganjali (2021)
Colorimetric biosensor for phenylalanine detection based on a paper using gold nanoparticles for phenylketonuria diagnosisMicrochemical Journal, 163
Scott Freeto, Donald Mason, Jie Chen, Robert Scott, Srinivas Narayan, M. Bennett (2007)
A rapid ultra performance liquid chromatography tandem mass spectrometric method for measuring amino acids associated with maple syrup urine disease, tyrosinaemia and phenylketonuriaAnnals of Clinical Biochemistry, 44
F. Ciolacu (2015)
A Simple, Sensitive and Highly Accurate Procedure for Plasma Phenylalanine Determination by HPLCActa Endocrinologica-bucharest, 11
E. Weetch, A. Macdonald (2006)
The determination of phenylalanine content of foods suitable for phenylketonuria.Journal of human nutrition and dietetics : the official journal of the British Dietetic Association, 19 3
P. Khadilkar, V. Kelkar, A. Khan (2013)
An optical biosensor employing phenylalanine ammonia lyase-immobilised films for phenylketonuria detection
N. Stasyuk, G. Gayda, Hasmik Yepremyan, A. Stępień, M. Gonchar (2017)
Fluorometric enzymatic assay of l-arginine.Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 170
Bridget Stroup, D. Ney, S. Murali, F. Rohr, S. Gleason, S. Calcar, H. Levy (2017)
Metabolomic Insights into the Nutritional Status of Adults and Adolescents with Phenylketonuria Consuming a Low-Phenylalanine Diet in Combination with Amino Acid and Glycomacropeptide Medical FoodsJournal of Nutrition and Metabolism, 2017
N Blau (2010)
1417The Lancet, 376
N. Blau, F. Spronsen, H. Levy (1961)
PhenylketonuriaThe Lancet, 376
H. Levy, C. Sarkissian, C. Scriver (2018)
Phenylalanine ammonia lyase (PAL): From discovery to enzyme substitution therapy for phenylketonuria.Molecular genetics and metabolism, 124 4
Longkuan Xiang, B. Moore (2005)
Biochemical Characterization of a Prokaryotic Phenylalanine Ammonia LyaseJournal of Bacteriology, 187
Baoting Sun, Zichen Wang, Xiaoyi Wang, Meng-xia Qiu, Zhijin Zhang, Ziyuan Wang, J. Cui, S. Jia (2020)
Paper-based biosensor based on phenylalnine ammonia lyase hybrid nanoflowers for urinary phenylalanine measurement.International journal of biological macromolecules
Selcen Aydaş, S. Şirin, B. Aslim (2016)
Biochemical analysis of Centaurea depressa phenylalanine ammonia lyase (PAL) for biotechnological applications in phenylketonuria (PKU)Pharmaceutical Biology, 54
Chang-Feng Li, L. Du, Hao Wu, Yi Chang (2011)
Determination of l-phenylalanine by cucurbit[7]uril sensitized fluorescence quenching methodChinese Chemical Letters, 22
Halit Arslan, K. Ünal, Elif Koyuncu, E. Yıldırım, F. Arslan (2020)
Development of a Novel Phenylalanine Biosensor for Diagnosis of PhenylketonuriaIEEE Sensors Journal, 20
Mei Zhang, Yong Zhang, Siqi Ren, Zunjian Zhang, Yongren Wang, Rui Song (2018)
Optimization of a Precolumn OPA Derivatization HPLC Assay for Monitoring of l-Asparagine Depletion in Serum during l-Asparaginase Therapy.Journal of chromatographic science, 56 9
Xiyue Xiong, Xiaoqi Sheng, Dan Liu, Ting Zeng, Ying Peng, Yichao Wang (2015)
A GC/MS-based metabolomic approach for reliable diagnosis of phenylketonuriaAnalytical and Bioanalytical Chemistry, 407
T. Arakawa, Tomoyuki Koshida, T. Gessei, K. Miyajima, D. Takahashi, H. Kudo, K. Yano, K. Mitsubayashi (2011)
Biosensor for L-phenylalanine based on the optical detection of NADH using a UV light emitting diodeMicrochimica Acta, 173
Zhu Wang, Yazhu Chen, Su Zhang, Zhen-sen Zhou (2005)
Investigation of a Phenylalanine-Biosensor System for Phenylketonuria Detection2005 IEEE Engineering in Medicine and Biology 27th Annual Conference
U. Ceglarek, P. Müller, B. Stach, P. Bührdel, J. Thiery, W. Kiess (2002)
Validation of the Phenylalanine/Tyrosine Ratio Determined by Tandem Mass Spectrometry: Sensitive Newborn Screening for Phenylketonuria, 40
Roman Kand'ár, Pavla Zakova (2009)
Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 877 30
Gregory Thiessen, R. Robinson, Kim Reyes, R. Monnat, Elain Fu (2015)
Conversion of a laboratory-based test for phenylalanine detection to a simple paper-based format and implications for PKU screening in low-resource settings.The Analyst, 140 2
Wayne Gardner, P. John (1991)
High-performance liquid chromatographic method to determine ammonium ion and primary amines in seawaterAnalytical Chemistry, 63
G. Neurauter, S. Scholl-Bürgi, Astrid Haara, S. Geisler, P. Mayersbach, H. Schennach, D. Fuchs (2013)
Simultaneous measurement of phenylalanine and tyrosine by high performance liquid chromatography (HPLC) with fluorescence detection.Clinical biochemistry, 46 18
N. Gerasimova, I. Steklova, T. Tuuminen (1989)
Fluorometric method for phenylalanine microplate assay adapted for phenylketonuria screening.Clinical chemistry, 35 10
Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations
Anubhuti Kawatra, Rakhi Dhankhar, A. Mohanty, P. Gulati (2020)
Biomedical applications of microbial phenylalanine ammonia lyase: Current status and future prospects.Biochimie
The measurement of phenylalanine in biological fluids for the diagnosis of phenylketonuria (PKU) in newborns and the monitoring/therapeutic drug monitoring of individuals with PKU are especially important. Owing to the importance of PKU monitoring in clinical medicine, a new fluorometric method was developed for the determination of L-phenylalanine in serum samples. This method is based on the relationship between phenylalanine ammonia-lyase (PAL) and o-phthalaldehyde (OPA). PAL catalyzes the conversion of phenylalanine to ammonia and trans-cinnamic acid. The formed ammonia reacts with OPA in the presence of sodium sulfite, giving a fluorescent product. The presence of sulfide in an alkaline environment prevents OPA from reacting with other amino acids while allowing it to react only with ammonia. Method characterization and optimization studies, such as the effects of pH, temperature, and interferents, were carried out. The amount of L-phenylalanine in a human serum sample was successfully determined by using the fluorescence emission intensity of the fluorescent product formed as a result of the reaction between OPA and ammonia. The linear range of the method is between 10 μM and 10 mM. The obtained result showed good agreement with the results of the biochemistry analysis laboratory. Recoveries of 95.41% and 73.39% were obtained for phenylalanine and ammonia, respectively. This PAL–OPA–based fluorometric method for phenylalanine is practical, easy to operate, low cost, highly sensitive, and selective and can also be used for the simultaneous determination of ammonia in human serum samples.Graphical Abstract[graphic not available: see fulltext]
Applied Biochemistry and Biotechnology – Springer Journals
Published: Mar 1, 2022
Keywords: L-Phenylalanine; Phenylalanine ammonia-lyase; o-Phthalaldehyde; Fluorometry; Phenylketonuria
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.