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Determination of 5′-mononucleotides in infant formula by capillary electrophoresis with ultraviolet detection

Determination of 5′-mononucleotides in infant formula by capillary electrophoresis with... A method for the simultaneous determination of five 5′-mononucleotides in infant formula by capillary electrophoresis (CE) with ultraviolet detection was developed. The analysis was performed using a bare fused-silica capillary (75 μm, i.d. × 50.2 cm; effective length, 40 cm) with a separation buffer consisting of 0.03 mol.L−1 sodium tetraborate and 0.08 mol.L−1 hydroxypropyl-β-cyclodextrin at pH 10.00. The samples were deproteinized with 0.05 mol.L−1 acetic acid. Limits of detection were between 0.8 and 1.0 mg.L−1 with an injection volume of 96 nL. The precision of the method was in the range of between 2.0% and 5.2% (n = 7). The recoveries at the concentrations studied (5 and 20 mg.L−1) were between 87.3% and 113.5%. The method was used for the analysis of five different kinds of infant formula, and the results were compared with the HPLC method. The CE method, which had high separation efficiency, consumed no solvent and could be used as an alternative to the HPLC method. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Dairy Science & Technology Springer Journals

Determination of 5′-mononucleotides in infant formula by capillary electrophoresis with ultraviolet detection

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Publisher
Springer Journals
Copyright
Copyright © 2011 by INRA and Springer Science+Business Media B.V.
Subject
Chemistry; Microbiology; Agriculture; Food Science
ISSN
1958-5586
eISSN
1958-5594
DOI
10.1007/s13594-011-0035-4
Publisher site
See Article on Publisher Site

Abstract

A method for the simultaneous determination of five 5′-mononucleotides in infant formula by capillary electrophoresis (CE) with ultraviolet detection was developed. The analysis was performed using a bare fused-silica capillary (75 μm, i.d. × 50.2 cm; effective length, 40 cm) with a separation buffer consisting of 0.03 mol.L−1 sodium tetraborate and 0.08 mol.L−1 hydroxypropyl-β-cyclodextrin at pH 10.00. The samples were deproteinized with 0.05 mol.L−1 acetic acid. Limits of detection were between 0.8 and 1.0 mg.L−1 with an injection volume of 96 nL. The precision of the method was in the range of between 2.0% and 5.2% (n = 7). The recoveries at the concentrations studied (5 and 20 mg.L−1) were between 87.3% and 113.5%. The method was used for the analysis of five different kinds of infant formula, and the results were compared with the HPLC method. The CE method, which had high separation efficiency, consumed no solvent and could be used as an alternative to the HPLC method.

Journal

Dairy Science & TechnologySpringer Journals

Published: Aug 3, 2011

References