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(1991)
Influence of genetic variants of β - lactoglobulin and κ - casein on milk composition and cheesemaking properties
L.A. Soria, G.M. Iglesias, M.J. Huguet (2003)
Mirande S.L., A PCR-RFLP test to detect allelic variants of the bovine kappa-casein geneAnim. Biotechnol., 14
E. Prinzenberg, I. Krause, G. Erhardt (1999)
SSCP analysis at the bovine CSN3 locus discriminates six alleles corresponding to known protein variants (A, B, C, E, F, G) and three new DNA polymorphisms (H, I, A1).Animal biotechnology, 10 1-2
(1991)
Influence of genetic variants of β-lactoglobulin and κ-casein on milk composition and cheesemaking properties, Lait
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
Alison Eenennaam, J. Medrano (1991)
Milk Protein Polymorphisms in California Dairy CattleJournal of Dairy Science, 74
S. Chessa, F. Chiatti, G. Ceriotti, A. Caroli, C. Consolandi, G. Pagnacco, Bianca Castiglioni (2007)
Development of a single nucleotide polymorphism genotyping microarray platform for the identification of bovine milk protein genetic polymorphisms.Journal of dairy science, 90 1
J. Mercier, G. Brignon, B. Ribadeau-Dumas (1973)
[Primary structure of bovine kappa B casein. Complete sequence].European journal of biochemistry, 35 2
B. Ribadeau-Dumas, R. Grappin (1989)
Milk protein analysisLait, 69
(1990)
Genotyping of bovine κ-casein loci following DNA sequence amplification, Bio/technology
J. Medrano, E. Cordova (1990)
Genotyping of Bovine Kappa-Casein Loci Following DNA Sequence AmplificationBio/Technology, 8
J. Mercier, F. Grosclaude, B. Ribadeau-Dumas (1970)
Structure primaire de la caséine β bovineFEBS Journal, 14
S. Kamiński, A. Ahman, A. Ruść, E. Wójcik, T. Malewski (2005)
MilkProtChip--a microarray of SNPs in candidate genes associated with milk protein biosynthesis--development and validation.Journal of applied genetics, 46 1
A. Summer, M. Malacarne, F. Martuzzi, P. Mariani (2002)
STRUCTURAL AND FUNCTIONAL CHARACTERISTICS OF MODENESE COW MILK IN PARMIGIANO-REGGIANO CHEESE PRODUCTION 1
V. Sheffield, D. Cox, L. Lerman, R. Myers (1989)
Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.Proceedings of the National Academy of Sciences of the United States of America, 86 1
J. Thompson, D. Higgins, T. Gibson (1994)
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.Nucleic acids research, 22 22
H. Farrell, R. Jiménez-Flores, G. Bleck, E. Brown, J. Butler, L. Creamer, C. Hicks, C. Hollar, K. Ng-Kwai-Hang, H. Swaisgood (1965)
Nomenclature of the proteins of cows' milk--sixth revision.Journal of dairy science, 87 6
J. Schaar, B. Hansson, H. Pettersson (1985)
Effects of genetic variants of κ-casein and β-lactoglobulin on cheesemakingJournal of Dairy Research, 52
L. Soria, G. Iglesias, M. Huguet, S. Mirande (2003)
A PCR‐RFLP Test to Detect Allelic Variants of the Bovine Kappa‐Casein GeneAnimal Biotechnology, 14
A. Barroso, S. Dunner, J. Cañón (1998)
Technical note: Detection of bovine kappa-casein variants A, B, C, and E by means of polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP).Journal of animal science, 76 6
(2002)
Structural and functional characteristics of Modenese cow milk in Parmigiano - Reggiano cheese production
A.L. Borresen-Dale, S. Lystad, A. Langerod (1997)
Temporal temperature gradient gel electrophoresis (TTGE) comparing with denaturating gradient gel electrophoresis (DGGE) and constant denaturating gel electrophoresis (CDGE) in mutation screeningBioradiation, 99
P. Boettcher, A. Caroli, Alessandra Stella, S. Chessa, E. Budelli, F. Canavesi, S. Ghiroldi, G. Pagnacco (2004)
Effects of casein haplotypes on milk production traits in Italian Holstein and Brown Swiss cattle.Journal of dairy science, 87 12
V. Rahali, J. Ménard (1991)
Influence des variants génétiques de la β-lactoglobuline et de la κ-caséine sur la composition du lait et son aptitude fromagèreLait, 71
(1985)
ff ects of genetic variants of κ - casein and β - lactoglobulin on cheese making
P. Udabage, I. Mckinnon, M. Augustin (2001)
Effects of mineral salts and calcium chelating agents on the gelation of renneted skim milk.Journal of dairy science, 84 7
The aim of this study was to develop and optimize a PCR-TTGE method for the detection of B κ-casein variant (CSN3*B), which is known to play a major role in cheese technology. The effects of the different κ-casein alleles on the quality and the quantity of cow’s milk have been widely reported and the availability of accurate and reliable protocols for the identification of the most common alleles is of great interest in breeding projects. In the present study a new genomic DNA extraction method from milk samples and a PCR-TTGE protocol to quickly identify A and B κ-casein allelic variants were developed. The DNA extraction method was fast and suitable for DNA amplification, while TTGE proved to be a powerful technique for discrimination of A and B alleles. This method was applied to 197 milk samples derived from Burlina, an Italian dairy cattle breed. CSN3*B allele frequency as detected by PCR-TTGE was 0.368. The development of this PCR-TTGE protocol could be used for future breeding programs to improve milk coagulation ability traits.
Dairy Science & Technology – Springer Journals
Published: May 21, 2011
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