Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Detection and molecular characterization of a 16SrI-F phytoplasma in potato showing purple top disease in Ecuador

Detection and molecular characterization of a 16SrI-F phytoplasma in potato showing purple top... Potato purple top (PPT) disease has been reported worldwide, including Canada, United States, Mexico, Russia and Pakistan. During surveys carried out in a recent epidemic in Ecuador of potato purple top disease phytoplasmas were identified in symptomatic potatoes as well as in some tissue cultured potato shoots by PCR/RFLP analyses and by sequencing and phylogenetic clustering. The majority of the samples resulted positive to phytoplasmas enclosed in the ribosomal subgroup 16SrI-F, for the first time detected in potato and in the American continent. Further studies will allow verification of the transmission and further presence in Ecuador of this phytoplasma associated with purple top disease of potatoes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

Detection and molecular characterization of a 16SrI-F phytoplasma in potato showing purple top disease in Ecuador

Download PDF
5 pages

Loading next page...
 
/lp/springer-journals/detection-and-molecular-characterization-of-a-16sri-f-phytoplasma-in-tqYfXKIj3U
Publisher
Springer Journals
Copyright
Copyright © 2018 by Australasian Plant Pathology Society Inc.
Subject
Life Sciences; Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1007/s13313-018-0557-9
Publisher site
See Article on Publisher Site

Abstract

Potato purple top (PPT) disease has been reported worldwide, including Canada, United States, Mexico, Russia and Pakistan. During surveys carried out in a recent epidemic in Ecuador of potato purple top disease phytoplasmas were identified in symptomatic potatoes as well as in some tissue cultured potato shoots by PCR/RFLP analyses and by sequencing and phylogenetic clustering. The majority of the samples resulted positive to phytoplasmas enclosed in the ribosomal subgroup 16SrI-F, for the first time detected in potato and in the American continent. Further studies will allow verification of the transmission and further presence in Ecuador of this phytoplasma associated with purple top disease of potatoes.

Journal

Australasian Plant Pathology – Springer Journals

Published: Apr 27, 2018

References

  • Phytoplasma collection. International Phytoplasmologists working group web
    Bertaccini, A
  • Avances en el diagnostic del agente causal del problema de punta morada en papa en Ecuador
    Castillo Carrillo, C; Buitrón, J; Insuasti, M; Castillo, N; Rivadeneira, J; Cuesta, X
  • Detection of ‘Candidatus Liberibacter solanacearum’ in the potato psyllid, Bactericera cockerelli (Sulc), by conventional and real-time PCR
    Crosslin, JM; Lin, H; Munyaneza, JE
  • Amplification of 16S ribosomal-RNA genes from culturable and nonculturable mollicutes
    Deng, SJ; Hiruki, C
  • Detección de ‘Ca. Liberibacter solanacearum’ y fitoplasmas en cultivo de papa (Solanum tuberosum L.) en el Valle de Toluca
    Gutiérrez-Ibáñez, AT; Sánchez-Pale, JR; Cerda, AL; Ramírez, DJF; Balbuena, AM; Alvarado, MA
  • Informe Técnico Anual. Fitoplasmas asociados a la punta morada de la papa en Ecuador. Instituto Nacional de Investigaciones Agropecuarias
  • Use of mycoplasma-like organism (MLOs) group specific oligonucleotide primers for nested-PCR assays to detect mixed-MLO infections in a single host plant
    Lee, I-M; Gundersen-Rindal, DE; Hammond, RD; Davis, RE
  • Revised classification scheme of phytoplasmas based on RFLP analyses of 16s rRNA and ribosomal protein gene sequences
    Lee, I-M; Gundersen-Rindal, DE; Davis, RE; Bartoszyk, IM
  • Clover proliferation group (16SrVI) subgroup a (16SrVI-a) phytoplasma is a probable causal agent of potato purple top disease in Washington and Oregon
    Lee, I-M; Bottner, KD; Munyaneza, JE; Secor, GA; Gudmestad, NC
  • A new ‘Candidatus Liberibacter’ species in Solanum tuberosum in New Zealand
    Liefting, LW; Perez-Egusquiza, ZC; Clover, GRG; Anderson, JAD
  • A new ‘Candidatus Liberibacter’ species associated with diseases of solanaceous crops
    Liefting, LW; Sutherland, PW; Ward, LI; Paice, KL; Weir, BS; Clover, GRG
  • Occurrence, distribution and detection of potato purple top phytoplasma disease in the Punjab (Pakistan)
    Nasir, MM; Mughal, SM; Khan, SM
  • Development of primers for improved PCR detection of the potato zebra chip pathogen, ‘Candidatus Liberibacter solanacearum’
    Ravindran, A; Levy, J; Pierson, E; Gross, DC
  • Genetic diversity and geographical distribution of phytoplasmas associated with potato purple top disease in Mexico
    Santos-Cervantes, ME; Chávez-Medina, JA; Acosta-Pardini, J; Flores-Zamora, GL; Mendez-Lozano, J; Leyva-Lopez, NE
  • Simultaneous detection of mixed ‘Candidatus Phytoplasma asteris’ and ‘Ca. Liberibacter solanacearum’infection in carrot
    Satta, E; Ramirez, AS; Paltrinieri, S; Contaldo, N; Benito, P; Poveda, JB; Bertaccini, A
  • Molecular and diagnostic procedures in mycoplasmology
    Schneider, B; Seemüller, E; Smart, CD; Kirkpatrick, BC
  • Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees
    Tamura, K; Nei, M
  • MEGA6: molecular evolutionary genetics analysis version 6.0
    Tamura, K; Stecher, G; Peterson, D; Filipski, A; Kumar, S