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Degradation of 2,4-Dinitrophenol by Free and Immobilized Cells of Rhodococcus erythropolis HL PM-1

Degradation of 2,4-Dinitrophenol by Free and Immobilized Cells of Rhodococcus erythropolis HL PM-1 The degradation of 2,4-dinitrophenol (2,4-DNP) by Rhodococcus erythropolis HL PM-1 was studied. The enzymes involved in 2,4-DNP degradation were inducible, and their resynthesis took place during the process. Cell immobilization by embedding into agar gels decreased the degrader activity. The maximum rates of 2,4-DNP degradation by free and immobilized cells were 10.0 and 5.4 nmol/min per mg cells, respectively. The concentration dependence of 2,4-DNP degradation was typical of substrate inhibition kinetics. The immobilized cells were used in a model reactor designed for 2,4-DNP biodegradation. Its maximum capacity was 0.45 nmol/min per mg cells at a volumetric flow rate of 20 h–1. The reactor operated for 14 days without losing capacity; its half-life equaled 16 days. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Degradation of 2,4-Dinitrophenol by Free and Immobilized Cells of Rhodococcus erythropolis HL PM-1

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References (10)

Publisher
Springer Journals
Copyright
Copyright © 2004 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Medical Microbiology; Biochemistry, general; Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1023/B:ABIM.0000025948.77233.dc
Publisher site
See Article on Publisher Site

Abstract

The degradation of 2,4-dinitrophenol (2,4-DNP) by Rhodococcus erythropolis HL PM-1 was studied. The enzymes involved in 2,4-DNP degradation were inducible, and their resynthesis took place during the process. Cell immobilization by embedding into agar gels decreased the degrader activity. The maximum rates of 2,4-DNP degradation by free and immobilized cells were 10.0 and 5.4 nmol/min per mg cells, respectively. The concentration dependence of 2,4-DNP degradation was typical of substrate inhibition kinetics. The immobilized cells were used in a model reactor designed for 2,4-DNP biodegradation. Its maximum capacity was 0.45 nmol/min per mg cells at a volumetric flow rate of 20 h–1. The reactor operated for 14 days without losing capacity; its half-life equaled 16 days.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Oct 18, 2004

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