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Cytogenetic and ITS-psbA-trnH Sequence Analysis for Phylogenetic Inference in Mucuna sp. of India

Cytogenetic and ITS-psbA-trnH Sequence Analysis for Phylogenetic Inference in Mucuna sp. of India This paper reports new chromosomal information and earliest proof on efficacy of nrITS and cp-psbA-trnH gene sequences for barcoding and phylogenetic studies in Mucuna sp. First cytological evidence on M. sempervirens and M. bracteata revealed symmetrical karyotype in both the species. Meiotic anomalies evident from the formation of chromosomal laggards and bridges in M. gigantea and M. atropurpurea and unreduced pollens in M. bracteata suggest evolutionary events that might have shaped annual species in this genus. Molecular investigations using nrITS and cp-psbA-trnH sequences revealed them to be phylogenetically informative. Vis-à-vis individual genes, combined sequences of ITS and psbA-trnH sequences offered reliable data for species delineation. The results summarized here are expected to galvanize molecular taxonomic studies and open-up newer means for answering phylogenetic questions in this genus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Tropical Plant Biology Springer Journals

Cytogenetic and ITS-psbA-trnH Sequence Analysis for Phylogenetic Inference in Mucuna sp. of India

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Springer Science+Business Media New York
Subject
Life Sciences; Plant Sciences; Plant Genetics & Genomics; Plant Breeding/Biotechnology; Plant Ecology; Transgenics
ISSN
1935-9756
eISSN
1935-9764
DOI
10.1007/s12042-015-9154-z
Publisher site
See Article on Publisher Site

Abstract

This paper reports new chromosomal information and earliest proof on efficacy of nrITS and cp-psbA-trnH gene sequences for barcoding and phylogenetic studies in Mucuna sp. First cytological evidence on M. sempervirens and M. bracteata revealed symmetrical karyotype in both the species. Meiotic anomalies evident from the formation of chromosomal laggards and bridges in M. gigantea and M. atropurpurea and unreduced pollens in M. bracteata suggest evolutionary events that might have shaped annual species in this genus. Molecular investigations using nrITS and cp-psbA-trnH sequences revealed them to be phylogenetically informative. Vis-à-vis individual genes, combined sequences of ITS and psbA-trnH sequences offered reliable data for species delineation. The results summarized here are expected to galvanize molecular taxonomic studies and open-up newer means for answering phylogenetic questions in this genus.

Journal

Tropical Plant BiologySpringer Journals

Published: Oct 17, 2015

References