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References (5)
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H. Berg (1986)
Immobilized Cells and Enzymes: A Practical Approach. : J. Woodward (Editor). IRL Press, Oxford, 1985, 177 pp., £13.00.Bioelectrochemistry and Bioenergetics, 15
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(1993)
Lekarstvennye sredstva (Drugs), Moscow: Meditsyna -
(1981)
Metody opredeleniya aktivnosti gidroliticheskikh fermentov ( Determination of Hydrolytic Enzyme Activity : Methods ) , Moscow : Legkaya i Pishchevaya Promst ' , -
(1981)
Metody opredeleniya aktivnosti gidroliticheskikh fermentov (Determination of Hydrolytic Enzyme Activity: Methods), Moscow -
M.D. Mashkovskii (1993)
Lekarstvennye sredstva (Drugs)
- Publisher
- Springer Journals
- Copyright
- Copyright © 2000 by Berdutina, Neklyudov, Ivankin, Karpo, Mitaleva
- Subject
- Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
- ISSN
- 0003-6838
- eISSN
- 1608-3024
- DOI
- 10.1007/BF02738043
- Publisher site
- See Article on Publisher Site
Abstract
The proteolytic activity and thermal stability of the enzyme complex of a cell suspension from pig and bovine pancreas glands was compared with those of pancreatin. The enzyme complex displayed the highest thermal stability and activity at 50°C. The kinetic constants, energies of activation and inactivation of the enzyme complex, and pH optimum (7.0 ± 0.1) at which this complex had the maximum proteolytic activity were determined. Pancreatin had a pH optimum of 8.0 ±0.1.
Journal
Applied Biochemistry and Microbiology – Springer Journals
Published: Oct 27, 2007