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Comparison of genomic profiles in human neuroblastic SH-SY5Y and substrate-adherent SH-EP cells using array comparative genomic hybridization

Comparison of genomic profiles in human neuroblastic SH-SY5Y and substrate-adherent SH-EP cells... Abstract Human neuroblastoma is the most commonly diagnosed solid tumor in children. The existence and development of S-type cells is important for the prognosis and malignant properties in neuroblastoma patients. However, their origin is controversial and the relationship between S- and N-type cells in neuroblastoma has not yet been clarified. To investigate the feasibility of inter-conversion and characteristic features between S-type cells lacking malignant potential and N-type cells having metastatic potential, the genomic profiles of neuroblastoma SH-EP (S-type) and SH-SY5Y (N-type) cells were compared at high resolution. Common gain segments (>10 Mb) between SH-EP and SH-SY5Y cells were observed at 1q21.1–q44 and 17q21.32–q25.3. The results of the fluorescent in situ hybridization (FISH) analysis showed good agreement with the array-CGH data. Genome-wide inspection of SH-EP and SH-SY5Y cells successfully identified not only common chromosomal aberrations but also genomic variations, suggesting that interconversion could not take place between S- and Ntype cells. The identified differences between less aggressive S-type cells (SH-EP) and highly aggressive neuroblastic N-type cells (SH-SY5Y) might be useful for understanding tumorigenicity and discovery of potential new markers in neuroblastoma. This is the first effort to compare genomic profiles between less aggressive S-type cells (SH-EP) and highly aggressive neuroblastic N-type cells (SH-SY5Y) at high resolution. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png BioChip Journal Springer Journals

Comparison of genomic profiles in human neuroblastic SH-SY5Y and substrate-adherent SH-EP cells using array comparative genomic hybridization

BioChip Journal , Volume 5 (2): 10 – Jun 1, 2011

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References (33)

Publisher
Springer Journals
Copyright
2011 The Korean BioChip Society and Springer-Verlag Berlin Heidelberg
ISSN
1976-0280
eISSN
2092-7843
DOI
10.1007/s13206-011-5210-4
Publisher site
See Article on Publisher Site

Abstract

Abstract Human neuroblastoma is the most commonly diagnosed solid tumor in children. The existence and development of S-type cells is important for the prognosis and malignant properties in neuroblastoma patients. However, their origin is controversial and the relationship between S- and N-type cells in neuroblastoma has not yet been clarified. To investigate the feasibility of inter-conversion and characteristic features between S-type cells lacking malignant potential and N-type cells having metastatic potential, the genomic profiles of neuroblastoma SH-EP (S-type) and SH-SY5Y (N-type) cells were compared at high resolution. Common gain segments (>10 Mb) between SH-EP and SH-SY5Y cells were observed at 1q21.1–q44 and 17q21.32–q25.3. The results of the fluorescent in situ hybridization (FISH) analysis showed good agreement with the array-CGH data. Genome-wide inspection of SH-EP and SH-SY5Y cells successfully identified not only common chromosomal aberrations but also genomic variations, suggesting that interconversion could not take place between S- and Ntype cells. The identified differences between less aggressive S-type cells (SH-EP) and highly aggressive neuroblastic N-type cells (SH-SY5Y) might be useful for understanding tumorigenicity and discovery of potential new markers in neuroblastoma. This is the first effort to compare genomic profiles between less aggressive S-type cells (SH-EP) and highly aggressive neuroblastic N-type cells (SH-SY5Y) at high resolution.

Journal

BioChip JournalSpringer Journals

Published: Jun 1, 2011

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