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Comparison of articular and auricular cartilages: decellularization, cell proliferation rate, and infiltration in scaffolds

Comparison of articular and auricular cartilages: decellularization, cell proliferation rate, and... PurposeVarious treatment approaches are applied to repair damaged cartilage. However, a developing field of tissue engineering holds a realistic promise to replace injured cartilage tissue using the patient’s cells. Using one type of chondrocytes to repair functionally different and far localized cartilage can be feasible from a clinical perspective. Toward this ultimate goal, we aimed to implement key protocols utilized in tissue engineering of articular (AR) and auricular (AU) cartilages.MethodsThe experiments were performed according to established protocols that include chondrocyte isolation, assessment of the cell proliferation rates, the degree of cell infiltration in three-dimensional scaffolds, and cartilage decellularization efficacy.ResultsThe data pointed to significant discrepancies in the size and in vitro chondrocytes proliferation rate isolated from distinct types of cartilage, with AR chondrocytes being 55% larger (p < 0.01) while having a slower rate of proliferation. Both collagen- and alginate-based scaffolds showed relevant properties for cell infiltration. Lastly, we have shown that the AR and AU cartilages are decellularized to a different degree (17 ± 5.5% vs. 42 ± 8.5%, p < 0.01) while using the same SDS-based decellularization protocol.ConclusionThis study will contribute to the global efforts to rebuild damaged cartilage with the help of tissue engineering. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Biomedical Engineering Springer Journals

Comparison of articular and auricular cartilages: decellularization, cell proliferation rate, and infiltration in scaffolds

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Publisher
Springer Journals
Copyright
Copyright © Sociedade Brasileira de Engenharia Biomedica 2021
ISSN
2446-4732
eISSN
2446-4740
DOI
10.1007/s42600-021-00141-8
Publisher site
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Abstract

PurposeVarious treatment approaches are applied to repair damaged cartilage. However, a developing field of tissue engineering holds a realistic promise to replace injured cartilage tissue using the patient’s cells. Using one type of chondrocytes to repair functionally different and far localized cartilage can be feasible from a clinical perspective. Toward this ultimate goal, we aimed to implement key protocols utilized in tissue engineering of articular (AR) and auricular (AU) cartilages.MethodsThe experiments were performed according to established protocols that include chondrocyte isolation, assessment of the cell proliferation rates, the degree of cell infiltration in three-dimensional scaffolds, and cartilage decellularization efficacy.ResultsThe data pointed to significant discrepancies in the size and in vitro chondrocytes proliferation rate isolated from distinct types of cartilage, with AR chondrocytes being 55% larger (p < 0.01) while having a slower rate of proliferation. Both collagen- and alginate-based scaffolds showed relevant properties for cell infiltration. Lastly, we have shown that the AR and AU cartilages are decellularized to a different degree (17 ± 5.5% vs. 42 ± 8.5%, p < 0.01) while using the same SDS-based decellularization protocol.ConclusionThis study will contribute to the global efforts to rebuild damaged cartilage with the help of tissue engineering.

Journal

Research on Biomedical Engineering – Springer Journals

Published: Apr 12, 2021

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