Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Comparative evaluation of the antimicrobial effect of electric plasma in textile between a standard strain of Klebsiella pneumoniae and a resistant clinical strain (KPC)

Comparative evaluation of the antimicrobial effect of electric plasma in textile between a... ObjectiveTo evaluate the antimicrobial effect of high-frequency electric plasma on a cotton textile article containing planktonic cells and biofilm formation of a standard strain and a multi-resistant clinical strain of Klebsiella pneumoniae carbapenemase (KPC), and to evaluate the integrity of the post textile fiber treatment, suggesting an alternative method to control this bacterium in hospital fabrics.MethodsBased on the American Association of Textile Chemists and Colorists – 100 methodology, inoculants of the standard strain of K. pneumoniae ATCC 4352 and a clinical strain of KPC isolated from hospital infection were prepared. These were used to contaminate 100% cotton fabric samples, and the antibacterial effect of electric plasma was evaluated at 2, 3, 4, and 5 min in planktonic cells and 4, 6, 8, and 10 min in a 48-h biofilm, assessing the antimicrobial effect by colony-forming units per milliliter count and the cell viability by MTT assay, respectively. The results were statistically analyzed using the GraphPad Prism 5 program, considering P <0.05 significant. The presence of biofilm in the fibers and the effect of electric plasma on their integrity was assessed by scanning electron microscopy (SEM).ResultsFor both strains, the percentage of reduction in planktonic cells ranged from 23.53 to 100%, with a significant reduction in all treatment times for both strains, except for the KPC strain in 2 min, with total elimination in 5 min. In addition, significant anti-biofilm activity was obtained after 8 and 10 min of treatment exclusively for the standard strain. There was no damage to the integrity of the textile fiber after the treatment.ConclusionElectric plasma is a promising physical agent, as long as the application time and intensity are respected, against planktonic cells and K. pneumoniae biofilm in cotton textiles, used in hospital garments. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Biomedical Engineering Springer Journals

Comparative evaluation of the antimicrobial effect of electric plasma in textile between a standard strain of Klebsiella pneumoniae and a resistant clinical strain (KPC)

Download PDF
11 pages

Loading next page...
 
/lp/springer-journals/comparative-evaluation-of-the-antimicrobial-effect-of-electric-plasma-2tie9XHt5d
Publisher
Springer Journals
Copyright
Copyright © Sociedade Brasileira de Engenharia Biomedica 2021
ISSN
2446-4732
eISSN
2446-4740
DOI
10.1007/s42600-021-00179-8
Publisher site
See Article on Publisher Site

Abstract

ObjectiveTo evaluate the antimicrobial effect of high-frequency electric plasma on a cotton textile article containing planktonic cells and biofilm formation of a standard strain and a multi-resistant clinical strain of Klebsiella pneumoniae carbapenemase (KPC), and to evaluate the integrity of the post textile fiber treatment, suggesting an alternative method to control this bacterium in hospital fabrics.MethodsBased on the American Association of Textile Chemists and Colorists – 100 methodology, inoculants of the standard strain of K. pneumoniae ATCC 4352 and a clinical strain of KPC isolated from hospital infection were prepared. These were used to contaminate 100% cotton fabric samples, and the antibacterial effect of electric plasma was evaluated at 2, 3, 4, and 5 min in planktonic cells and 4, 6, 8, and 10 min in a 48-h biofilm, assessing the antimicrobial effect by colony-forming units per milliliter count and the cell viability by MTT assay, respectively. The results were statistically analyzed using the GraphPad Prism 5 program, considering P <0.05 significant. The presence of biofilm in the fibers and the effect of electric plasma on their integrity was assessed by scanning electron microscopy (SEM).ResultsFor both strains, the percentage of reduction in planktonic cells ranged from 23.53 to 100%, with a significant reduction in all treatment times for both strains, except for the KPC strain in 2 min, with total elimination in 5 min. In addition, significant anti-biofilm activity was obtained after 8 and 10 min of treatment exclusively for the standard strain. There was no damage to the integrity of the textile fiber after the treatment.ConclusionElectric plasma is a promising physical agent, as long as the application time and intensity are respected, against planktonic cells and K. pneumoniae biofilm in cotton textiles, used in hospital garments.

Journal

Research on Biomedical Engineering – Springer Journals

Published: Dec 1, 2021

Keywords: Textiles; Hospital-acquired infection; Electric plasma; Klebsiella pneumoniae; KPC

References

  • Wastewater treatment by oxidation with ozone
    Almeida, E
  • Role of antibiotic penetration limitation in Klebsiella pneumoniae biofilm resistance to ampicillin and ciprofloxacin
    Anderl, JN; Franklin, MJ; Stewart, PS
  • Antibacterial efficacy and mechanisms of action of low power atmospheric pressure cold plasma: membrane permeability, biofilm penetration and antimicrobial sensitization
    Brun, P
  • Antimicrobial and antibiofilm activity of Lys-[Trp6] hy-a1 combined with ciprofloxacin against Gram-negative bacteria
    Carneiro, VA
  • Outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in an intermediate-risk neonatal unit linked to onychomycosis in a healthcare worker
    Cassettari, VC
  • Inhibiting biofilm formation by Klebsiella pneumoniae B5055 using an iron antagonizing molecule and a bacteriophage
    Chhibber, S; Nag, D; Bansal, S
  • The role of the laundry in the recontamination of washed bedding
    Church, BD; Loosli, CG
  • Application of a combination of neutral red and amido black staining for rapid, reliable cytotoxicity testing of biomaterials
    Ciapetti, G
  • Bacterial biofilms: a common cause of persistent infections
    Costerton, JW; Stewart, PS; Greenberg, EP
  • Controle de infecção hospitalar: histórico e papel do estado
    De Oliveira, R; Maruyama, SAT
  • Comparative study of Candida albicans inactivation by nonthermal plasma on stainless steel with and without diamond-like carbon film
    De Oliveira, SMM
  • La resistencia bacteriana en el contexto de la infección hospitalaria
    De Queiroz, NS
  • Hospital-acquired infections in the adult intensive care unit—epidemiology, antimicrobial resistance patterns, and risk factors for acquisition and mortality
    Despotovic, A
  • Biofilms: survival mechanisms of clinically relevant microorganisms
    Donlan, RM; Costerton, JW
  • Application of post-discharge region of atmospheric pressure argon and air plasma jet in the contamination control of Candida albicans biofilms
    Doria, ACOC
  • Self-cleaned photoluminescent viscose fabric incorporated lanthanide-organic framework (Ln-MOF)
    Emam, HE; Abdelhamid, HN; Abdelhameed, RM
  • Inhibition of biofilms by non-thermal plasma treated novel solutions
    Ercan, UK
  • Multi-agent modelling and simulation of hospital acquired infection propagation dynamics by contact transmission in hospital wards
    Esposito, D
  • Kpi, a chaperone-usher pili system associated with the worldwide-disseminated high-risk clone Klebsiella pneumoniae ST-15
    Gato, E
  • Continuous evolution: perspective on the epidemiology of carbapenemase resistance among enterobacterales and other gram-negative bacteria
    Hansen, GT
  • Synergy testing by E-test and microdilution checkerboard for fosfomycin combined with tigecycline against KPC-producing Klebsiella pneumoniae
    Huang, L; Wang, M; Sun, L
  • Synergism in biofilm formation between Salmonella enteritidis and a nitrogen-fixing strain of Klebsiella pneumoniae
    Jones, K; Bradshaw, SB
  • The significant influence of bacterial reaction on physico-chemical property changes of biodegradable natural and synthetic polymers using Escherichia coli
    Kang, C
  • A review on mechanism of action, resistance, synergism, and clinical implications of mupirocin against Staphylococcus aureus
    Khoshnood, S
  • Plasma medicine: an introductory review
    Kong, MG
  • The effect of liquid target on a nonthermal plasma jet—imaging, electric fields, visualization of gas flow and optical emission spectroscopy
    Kovačević, VV
  • The antibacterial effect of non-thermal atmospheric pressure plasma treatment of titanium surfaces according to the bacterial wall structure
    Lee, MJ
  • Capsular polysaccharide types and virulence-related traits of epidemic KPC-producing Klebsiella pneumoniae isolates in a Chinese university hospital
    Liu, Y
  • Dynamic behavior of plasma and molten pool of pure titanium during hyperbaric laser welding
    Long, J
  • Efeito bactericida do gerador de alta frequência na cultura de Staphylococcus aureus
    Martins, A
  • A meta-analysis of in vitro antibiotic synergy against Acinetobacter baumannii
    March, GA; Bratos, MA
  • Infecção hospitalar em pacientes cirúrgicos de hospital universitário
    Medeiros, AC
  • Cold atmospheric pressure plasma elimination of clinically important single-and mixed-species biofilms
    Modic, M
  • Polymeric materials with antimicrobial activity
    Muñoz-bonilla, A; Fernández-garcía, M
  • On the history of plasma treatment and comparison of microbiostatic efficacy of a historical high-frequency plasma device with two modern devices
    Napp, J
  • Does antibiotic resistance impair plasma susceptibility of multi-drug resistant clinical isolates of enterococci in vitro?
    Napp, M; Von Podewils, S; Klare, I
  • Survival of enterococci and staphylococci on hospital fabrics and plastic
    Neely, AN; Maley, MP
  • Inhibitor resistance in the KPC-2 β-lactamase, a preeminent property of this class A β-lactamase
    Papp-wallace, KM
  • Effects of non-thermal atmospheric pressure plasma on palatal wound healing of free gingival grafts: a randomized controlled clinical trial
    Pekbağrıyanık, T; Dadas, FK; Enhoş, Ş
  • A infecção hospitalar e suas implicações para o cuidar da enfermagem
    Pereira, MS
  • Carbapenemase-producing Klebsiella pneumoniae, a key pathogen set for global nosocomial dominance
    Pitout, JDD; Nordmann, P; Poirel, L
  • Ros from physical plasmas: redox chemistry for biomedical therapy
    Privat-maldonado, A
  • Preformed antibiotic-loaded cement spacers for two-stage revision of infected total hip arthroplasty
    Romanò, CL
  • Effective antibacterial adhesive coating on cotton fabric using ZnO nanorods and chalcone
    Sivakumar, PM
  • Inactivation of single strains of Listeria monocytogenes and Salmonella typhimurium planktonic cells biofilms with plasma activated liquids
    Smet, C
  • Exposure of Salmonella biofilms to antibiotic concentrations rapidly selects resistance with collateral tradeoffs
    Trampari, E
  • Infecção hospitalar e causas múltiplas de morte
    Turrini, RNT; Santo, AH
  • Sterilization of endodontic files with oxygen plasma
    Ueno, M
  • Novel carbapenem-hydrolyzing β-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae
    Yigit, H
  • In vitro and in vivo bactericidal activity of ceftazidime-avibactam against Carbapenemase–producing Klebsiella pneumoniae
    Zhang, W
  • Synergistic antibacterial effects of treatments with low temperature plasma jet and pulsed electric fields
    Zhang, Q