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Comparative Cell Shape and Diffusional Water Permeability of Red Blood Cells from Indian Elephant (Elephas maximus) and Man (Homo sapiens)

Comparative Cell Shape and Diffusional Water Permeability of Red Blood Cells from Indian Elephant... Red blood cells (RBC) from an Indian elephant (Elephas maximus) were studied by light microscopy (LM), scanning electron microscopy (SEM) and a new nuclear magnetic resonance (NMR) ‘imaging’ method based on the translational diffusion of water, NMR q-space analysis. Also, the transmembrane diffusional permeability, P d of water in RBC was measured by using a Mn2+-doping NMR technique, taking human RBC as a reference. The main diameter of the elephant RBC was measured as 9.3 ± 0.7 μm by LM, 9.3 ± 0.7 μm by ‘shrinkage-corrected’ SEM, and 9.3 ± 0.4 μm by q-space anlaysis. The value is ∼1.4 μm larger than that for the human RBC. The values of P d were, in the case of elephant RBC, 3.2 × 10−3 cm/s at 25 °C, 3.9 × 10−3 cm/s at 30 °C, 5.2 × 10−3 cm/s at 37 °C and 6.5 × 10−3 cm/s at 42 °C; all values were significantly lower than the corresponding values of P d for human RBC, namely 4.3 × 10−3 cm/s at 25 °C, 5.2 × 10−3 cm/s at 30 °C, 6.1 × 10−3 cm/s at 37 °C, 7.8 × 10−3 cm/s at 42°C. The maximal inhibition of P d (56%) was reached in 30 min at 37 °C with 2 mm p-chloromercuribenzene sulphonate (PCMBS) for both species of RBC. The basal permeability to water at 37 °C was estimated to be 2.3 × 10−3 cm/s for elephant and 2.6 × 10−3 cm/s for human RBC. The values of the activation energy for water permeability (E a,d ) was significantly higher for elephant RBC (31.9 kJ/mol) than for human RBC (25.9 kJ/mol). This indicated that features other than the number of transporters per cell are likely to be important in defining the differences in water permeability in the RBC from the two species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Comparative Cell Shape and Diffusional Water Permeability of Red Blood Cells from Indian Elephant (Elephas maximus) and Man (Homo sapiens)

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References (29)

Publisher
Springer Journals
Copyright
Copyright © 2000 by Springer-Verlag London Limited
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1433-2973
DOI
10.1007/s005800070020
Publisher site
See Article on Publisher Site

Abstract

Red blood cells (RBC) from an Indian elephant (Elephas maximus) were studied by light microscopy (LM), scanning electron microscopy (SEM) and a new nuclear magnetic resonance (NMR) ‘imaging’ method based on the translational diffusion of water, NMR q-space analysis. Also, the transmembrane diffusional permeability, P d of water in RBC was measured by using a Mn2+-doping NMR technique, taking human RBC as a reference. The main diameter of the elephant RBC was measured as 9.3 ± 0.7 μm by LM, 9.3 ± 0.7 μm by ‘shrinkage-corrected’ SEM, and 9.3 ± 0.4 μm by q-space anlaysis. The value is ∼1.4 μm larger than that for the human RBC. The values of P d were, in the case of elephant RBC, 3.2 × 10−3 cm/s at 25 °C, 3.9 × 10−3 cm/s at 30 °C, 5.2 × 10−3 cm/s at 37 °C and 6.5 × 10−3 cm/s at 42 °C; all values were significantly lower than the corresponding values of P d for human RBC, namely 4.3 × 10−3 cm/s at 25 °C, 5.2 × 10−3 cm/s at 30 °C, 6.1 × 10−3 cm/s at 37 °C, 7.8 × 10−3 cm/s at 42°C. The maximal inhibition of P d (56%) was reached in 30 min at 37 °C with 2 mm p-chloromercuribenzene sulphonate (PCMBS) for both species of RBC. The basal permeability to water at 37 °C was estimated to be 2.3 × 10−3 cm/s for elephant and 2.6 × 10−3 cm/s for human RBC. The values of the activation energy for water permeability (E a,d ) was significantly higher for elephant RBC (31.9 kJ/mol) than for human RBC (25.9 kJ/mol). This indicated that features other than the number of transporters per cell are likely to be important in defining the differences in water permeability in the RBC from the two species.

Journal

Comparative Clinical Pathology – Springer Journals

Published: Jul 1, 2000

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